An aseptic coupling assembly, includes a first connector and a second connector. The first connector includes a first housing, a first valve member, and a first spring that biases the first valve member towards the second end of the first housing to a first closed position. The second connector includes a second housing, a second valve member, and a second spring that biases the second valve member towards the second end of the second housing to a second closed position. The first valve member is slidable away from the first closed position within the first housing to compress the first spring and the second valve member is slidable away from the second closed position within the second housing to compress the second spring. The coupling assembly further includes a seal that prevents fluid from contacting the first spring and the second spring.