- 专利标题: CELL PRESERVATION METHOD
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申请号: US17915415申请日: 2021-03-30
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公开(公告)号: US20230132689A1公开(公告)日: 2023-05-04
- 发明人: Yasutomo YANAGITA , Chugo RINOIE , Takefumi ISHIDAO , Itsunari MINAMI
- 申请人: Cell Exosome Therapeutics Inc.
- 申请人地址: JP Tokyo
- 专利权人: Cell Exosome Therapeutics Inc.
- 当前专利权人: Cell Exosome Therapeutics Inc.
- 当前专利权人地址: JP Tokyo
- 优先权: JP2020-063821 20200331
- 国际申请: PCT/JP2021/013685 WO 20210330
- 主分类号: A01N1/02
- IPC分类号: A01N1/02 ; C12N5/0775 ; A61K35/28 ; A61K47/10 ; A61K47/20 ; A61K9/08
摘要:
The purpose of the present invention is to obtain a cell suspension with a low concentration of cryoprotectant. This method of preserving cells used comprises the steps of (a) enriching cells from a cell suspension containing the cells and a cryoprotective solution to generate an enriched fraction, and (b) freezing the enriched fraction to prepare a frozen material. It is also possible to use a method of producing a cell suspension, comprising the steps of (a) enriching cells from a cell suspension containing the cells and a cryoprotective solution to generate an enriched fraction, (b) freezing the enriched fraction to prepare a frozen material, (c) thawing the frozen material to prepare a thawed material, and (d) mixing the thawed material and a solution to produce a cell suspension.