The present disclosure provides a non-contact fatigue detection system and method based on rPPG. The system and method adopt multi-thread synchronous communication for real-time acquisition and processing of rPPG signal, enabling fatigue status detection. In this setup, the first thread handles real-time rPPG data capture, storage and concatenation, while the second thread conducts real-time analysis and fatigue detection of rPPG data. Through a combination of skin detection and LUV color space conversion, rPPG raw signal extraction is achieved, effectively eliminating interference from internal and external environmental facial noise; Subsequently, an adaptive multi-stage filtering process enhances the signal-to-noise ratio, and a multi-dimensional fusion CNN model ensures accurate detection of respiration and heart rate. The final step involves multi-channel data fusion of respiration and heartbeats, succeeding in not only learning person-independent features for fatigue detection but also detecting early fatigue with very high accuracy.