Invention Publication
- Patent Title: PUMA1 POLYMERASES AND USES THEREOF
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Application No.: US18448548Application Date: 2023-08-11
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Publication No.: US20240101978A1Publication Date: 2024-03-28
- Inventor: Lorita BOGHOSPOR , Yufeng QIAN
- Applicant: 10x Genomics, Inc.
- Applicant Address: US CA Pleasanton
- Assignee: 10x Genomics, Inc.
- Current Assignee: 10x Genomics, Inc.
- Current Assignee Address: US CA Pleasanton
- Main IPC: C12N9/12
- IPC: C12N9/12 ; C12N15/63 ; C12Q1/6844

Abstract:
The present disclosure relates in some aspects to recombinant polymerases, variant PumA1 polymerases, and compositions thereof. Also provided herein are methods of using the recombinant polymerases and/or variant PumA1 polymerases for nucleic acid amplification (e.g., rolling circle amplification). In some aspects, the compositions and methods disclosed herein provide more robust amplification (e.g., RCA) reactions for improved in vitro and in situ analysis.
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