公开(公告)号：US20230374573A1

公开(公告)日：2023-11-23

申请号：US18127582

申请日：2023-03-28

Applicant: 10x Genomics, Inc.

Inventor： Yufeng QIAN ,  Shankar SHASTRY

IPC: C12Q1/6844 ,  C12N9/22 ,  C12Q1/44

CPC classification number: C12Q1/6844 ,  C12N9/22 ,  C12Y301/26004 ,  C12Q1/44

Abstract: The present disclosure relates in some aspects to methods for analyzing a target RNA in a biological sample, such as detection of a sequence of interest in an RNA. In some aspects, provided herein are methods of using an RNase such as RNase H to provide in situ RNA detection methods having high sensitivity, efficiency, and/or specificity.

公开(公告)号：US20240368567A1

公开(公告)日：2024-11-07

申请号：US18744254

申请日：2024-06-14

Applicant: 10x Genomics, Inc.

Inventor： Derek Hunter VALLEJO ,  Sonya A. CLARK ,  Yufeng QIAN ,  Lorita BOGHOSPOR ,  Shankar SHASTRY

IPC: C12N9/12 ,  C12N9/10

Abstract: The disclosure provides recombinant reverse transcriptases comprising one or more DNA binding domains conjugated to an engineered reversed transcriptase that have been modified to exhibit one or more altered reverse transcriptase related activities such as but not limited to altered template switching efficiency, altered transcription efficiency or both. The disclosure further provides compositions and kits comprising the recombinant reverse transcriptase enzymes and methods of producing, amplifying or sequencing nucleic acid molecules using these fusion reverse transcriptase enzymes.

公开(公告)号：US20240351041A1

公开(公告)日：2024-10-24

申请号：US18634751

申请日：2024-04-12

Applicant: 10x Genomics, Inc.

Inventor： Cheyenne CHRISTOPHERSON ,  Justin COSTA ,  Shalini GOHIL ,  Hong CHEN ,  Yufeng QIAN ,  Minkyung PARK ,  Xiaoyang CAI ,  Hiroshi SASAKI ,  Naveed BAKH

IPC: B01L3/00

CPC classification number: B01L3/527 ,  B01L2200/025 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/12

Abstract: The present disclosure provides systems and methods for reagent storage. System may include one or more substrates comprising a partition. The partition may be configured to retain a first reagent separate from a second reagent. The partition may include a seal disposed between the first and second reagent. The seal may be configured to maintain the first reagent separate from the second reagent. The first and second reagent may be disposed in a first layer and a second layer, respectively, of frozen material. The first layer and second layer of frozen material may maintain the first reagent separate from the second reagent.

公开(公告)号：US20240101978A1

公开(公告)日：2024-03-28

申请号：US18448548

申请日：2023-08-11

Applicant: 10x Genomics, Inc.

Inventor： Lorita BOGHOSPOR ,  Yufeng QIAN

IPC: C12N9/12 ,  C12N15/63 ,  C12Q1/6844

CPC classification number: C12N9/1252 ,  C12N15/63 ,  C12Q1/6844 ,  C12Y207/07007

Abstract: The present disclosure relates in some aspects to recombinant polymerases, variant PumA1 polymerases, and compositions thereof. Also provided herein are methods of using the recombinant polymerases and/or variant PumA1 polymerases for nucleic acid amplification (e.g., rolling circle amplification). In some aspects, the compositions and methods disclosed herein provide more robust amplification (e.g., RCA) reactions for improved in vitro and in situ analysis.