- 专利标题: METHOD FOR PRODUCING DOPAMINERGIC NEURON PROGENITOR CELL
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申请号: US18539589申请日: 2023-12-14
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公开(公告)号: US20240117304A1公开(公告)日: 2024-04-11
- 发明人: Jun TAKAHASHI , Daisuke DOI , Kenji YOSHIDA , Atsushi KUWAHARA , Masayo TAKAHASHI
- 申请人: KYOTO UNIVERSITY , SUMITOMO PHARMA CO., LTD.
- 申请人地址: JP Kyoto-shi
- 专利权人: KYOTO UNIVERSITY,SUMITOMO PHARMA CO., LTD.
- 当前专利权人: KYOTO UNIVERSITY,SUMITOMO PHARMA CO., LTD.
- 当前专利权人地址: JP Kyoto-shi; JP Osaka-shi
- 优先权: JP 16086499 2016.04.22
- 主分类号: C12N5/0793
- IPC分类号: C12N5/0793 ; A61K35/30 ; C12N5/074
摘要:
A cell population comprising Corin- and/or Lrtm1-positive cells was produced by the following steps (1) and (2), from which Corin positive and/or Lrtm1 positive cells are collected using a substance that binds to Corin and/or a substance that binds to Lrtm1, and dopaminergie neuron progenitor cells are produced by performing suspension culture of the Corin positive and/or Lrtm1 positive cells in a culture solution containing one or more nutritional factors:
(1) a step of performing adhesion culture of pluripotent stem cells in a medium for maintaining undifferentiated state containing a Sonic hedgehog (SHH) signal stimulant, and an undifferentiated state-maintaining factor in the absence of feeder cells but in the presence of an extracellular matrix, and
(2) a step of culturing the cell population obtained in the step (1) in a culture solution containing one or more differentiation-inducing factors.
(1) a step of performing adhesion culture of pluripotent stem cells in a medium for maintaining undifferentiated state containing a Sonic hedgehog (SHH) signal stimulant, and an undifferentiated state-maintaining factor in the absence of feeder cells but in the presence of an extracellular matrix, and
(2) a step of culturing the cell population obtained in the step (1) in a culture solution containing one or more differentiation-inducing factors.
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