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1.发明公开公开(公告)号：US20240279602A1
公开(公告)日：2024-08-22
申请号：US18569736
申请日：2022-06-16
发明人： Jun TAKAHASHI , Daisuke DOI , Megumi IKEDA
IPC分类号： C12N5/0793 , A61K35/30 , C12Q1/68
CPC分类号： C12N5/0619 , A61K35/30 , C12Q1/68 , C12N2500/38 , C12N2501/01 , C12N2501/13 , C12N2506/45
摘要： The present disclosure provides a cerebral organoid derived from a human pluripotent stem cell, a cell aggregate including a cerebral cortical cell, and a method for producing any of them, each being useful for regenerative therapy.
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公开(公告)号：US20240117304A1
公开(公告)日：2024-04-11
申请号：US18539589
申请日：2023-12-14
IPC分类号： C12N5/0793 , A61K35/30 , C12N5/074
CPC分类号： C12N5/0619 , A61K35/30 , C12N5/0696 , C12N2500/38 , C12N2501/115 , C12N2501/119 , C12N2501/13 , C12N2501/15 , C12N2501/155 , C12N2501/41 , C12N2501/727 , C12N2501/999 , C12N2506/45 , C12N2533/90
摘要： A cell population comprising Corin- and/or Lrtm1-positive cells was produced by the following steps (1) and (2), from which Corin positive and/or Lrtm1 positive cells are collected using a substance that binds to Corin and/or a substance that binds to Lrtm1, and dopaminergie neuron progenitor cells are produced by performing suspension culture of the Corin positive and/or Lrtm1 positive cells in a culture solution containing one or more nutritional factors:
(1) a step of performing adhesion culture of pluripotent stem cells in a medium for maintaining undifferentiated state containing a Sonic hedgehog (SHH) signal stimulant, and an undifferentiated state-maintaining factor in the absence of feeder cells but in the presence of an extracellular matrix, and
(2) a step of culturing the cell population obtained in the step (1) in a culture solution containing one or more differentiation-inducing factors.-
3.发明申请公开(公告)号：US20190270965A1
公开(公告)日：2019-09-05
申请号：US16141548
申请日：2018-09-25
申请人： Kyoto University
发明人： Jun TAKAHASHI , Asuka Morizane
IPC分类号： C12N5/0735 , C12N5/074
摘要： The present invention provides a method for inducing differentiation of pluripotent stem cells into neural precursor cells, comprising culturing the pluripotent stem cells in the presence of a small, molecule BMP inhibitor, and induced neural precursor cells prepared by this method.
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公开(公告)号：US20180094241A1
公开(公告)日：2018-04-05
申请号：US15565984
申请日：2016-04-14
申请人： KYOTO UNIVERSITY
发明人： Jun TAKAHASHI , Makoto MOTONO , Yoshihiko IOROI
IPC分类号： C12N5/0793
CPC分类号： C12N5/0619 , A61K35/00 , A61K35/30 , A61K35/545 , C12N2501/115 , C12N2501/15 , C12N2501/155 , C12N2501/415 , C12N2506/02 , C12N2506/08 , C12N2506/13 , C12N2506/45
摘要： Provided is a method for producing cerebral cortex neurons from pluripotent stem cells.Provided is a method for producing cerebral cortex neurons from pluripotent stem cells, comprising (i) a step of performing a suspension culture of pluripotent stem cells in a culture medium containing a TGFβ inhibitor, bFGF, a Wnt inhibitor, and a BMP inhibitor, (ii) a step of performing a suspension culture of the cells obtained in the step (i) in a culture medium containing a Wnt inhibitor and a BMP inhibitor, and (iii) a step of further culturing the cells obtained in the step (ii).
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公开(公告)号：US20190112575A1
公开(公告)日：2019-04-18
申请号：US16095092
申请日：2017-04-24
IPC分类号： C12N5/0793 , C12N5/074
摘要： A cell population comprising Corin- and/or Lrtm1-positive cells was produced by the following steps (1) and (2), from which Corin positive and/or Lrtm1 positive cells are collected using a substance that binds to Corin and/or a substance that binds to Lrtm1, and dopaminergic neuron progenitor cells are produced by performing suspension culture of the Corin positive and/or Lrtm1 positive cells in a culture solution containing one or more nutritional factors: (1) a step of performing adhesion culture of pluripotent stem cells in a medium for maintaining undifferentiated state containing a Sonic hedgehog (SHH) signal stimulant, and an undifferentiated state-maintaining factor in the absence of feeder cells but in the presence of an extracellular matrix, and (2) a step of culturing the cell population obtained in the step (1) in a culture solution containing one or more differentiation-inducing factors.
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公开(公告)号：US20240033332A1
公开(公告)日：2024-02-01
申请号：US18257143
申请日：2021-12-16
申请人： KYOTO UNIVERSITY
发明人： Jun TAKAHASHI , Bumpei SAMATA
CPC分类号： A61K38/47 , A61K38/16 , C12Y302/01017 , A61K38/17 , C12Y304/23005 , A61P25/00 , C12Y304/23 , A61K38/488
摘要： Developing an agent for activating a neuron which activates a brain neuron. The agent is suitable for promoting cell transplantation for a neural disease, survival of a transplanted cell, differentiation of the transplanted cell into a neuronal cell, and/or maturation of the neuron. The agent for activating the neuron contains a protein selected from the group consisting of Lysozyme (LYZ), Secreted phosphoprotein 1 (SPP1), Progranulin (PGrn), Cathepsin D (Ctsd), Cathepsin S (Ctss), Apolipoprotein D (Apod), Sparc, CD52, Macrophage expressed gene 1 (MPEG1), C-C motif chemokine ligand 2 (CCL2), Cystatin F (CST7), Ras family small GTPase 2 (RAC2), Serpin family A member 3 (SERPINA3), Anosmin 1 (ANOS1), Kallikrein-related peptidase 6 (KLK6), Alpha-2-macroglobulin (A2M), GM14295, Galectin 3-binding protein (LGALS3BP), Apolipoprotein C1 (APOC1), and orthologs thereof, or an active fragment thereof, or a nucleic acid encoding the protein or active fragment thereof, and an agent for assisting cell transplantation containing the agent.
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