The present disclosure relates to a manufacturing method of a waterproof glove. In more detail, the manufacturing method of a waterproof glove includes: a membrane glove manufacturing step of manufacturing a glove using a polyurethane membrane sheet applied with a hot-melt adhesive; a lining glove inserting step of inserting a lining glove into the membrane glove manufactured to the membrane glove manufacturing step; a membrane glove inserting step of inserting the membrane glove with the lining glove inserted through the lining glove inserting step into an outshell glove; and a thermal bonding step of bonding the lining glove, the membrane glove, and the outshell glove by heating the outshell glove with the membrane glove inserted through the membrane glove inserting step.