Provided are a color interpolation method and an image acquisition device for a multispectral filter array. The color interpolation method includes acquiring a raw multispectral image on the basis of a multispectral filter array, generating a guide image for color interpolation of the raw multispectral image, generating a difference image between the raw multispectral image and the guide image, generating a demosaiced difference image by performing color interpolation based on the difference image, and generating a demosaiced multispectral image based on the demosaiced difference image and the guide image.