A display substrate, a method for manufacturing the display substrate, and a display device are provided. The display substrate includes: a base substrate; a metal layer on the base substrate, including a copper layer having a first thickness; an anti-oxidation layer on a side of the metal layer away from the base substrate, having a second thickness; and a light emitting diode on a side of the anti-oxidation layer away from the base substrate, including at least one electrode electrically connected to the metal layer, where the first thickness of the metal layer is larger than the second thickness of the anti-oxidation layer.