Invention Application
- Patent Title: ENGINEERED POLYMERASES WITH IMPROVED THERMAL STABILITY
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Application No.: US18975633Application Date: 2024-12-10
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Publication No.: US20250101390A1Publication Date: 2025-03-27
- Inventor: Jendrik BOOTHBY-HENTSCHEL , Michael KLEIN , Mark AMBROSO , Tyler LOPEZ , Matthew KELLINGER , Virginia SAADE , Ramkrishna ADHIKARY
- Applicant: Element Biosciences, Inc.
- Applicant Address: US CA San Diego
- Assignee: Element Biosciences, Inc.
- Current Assignee: Element Biosciences, Inc.
- Current Assignee Address: US CA San Diego
- Main IPC: C12N9/12
- IPC: C12N9/12 ; C12N15/11 ; C12Q1/48 ; C12Q1/6869

Abstract:
Provided herein are engineered variants of archaeal polymerases that exhibit exonuclease-minus activity, enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, improved uracil-tolerance and/or reduce sequence-specific errors in polymerase-catalyzed nucleotide binding and extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases and forming binding complexes, and uses for conducting nucleic acid sequencing reactions.
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