公开(公告)号：US20250101390A1

公开(公告)日：2025-03-27

申请号：US18975633

申请日：2024-12-10

Applicant: Element Biosciences, Inc.

Inventor： Jendrik BOOTHBY-HENTSCHEL ,  Michael KLEIN ,  Mark AMBROSO ,  Tyler LOPEZ ,  Matthew KELLINGER ,  Virginia SAADE ,  Ramkrishna ADHIKARY

IPC: C12N9/12 ,  C12N15/11 ,  C12Q1/48 ,  C12Q1/6869

Abstract: Provided herein are engineered variants of archaeal polymerases that exhibit exonuclease-minus activity, enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, improved uracil-tolerance and/or reduce sequence-specific errors in polymerase-catalyzed nucleotide binding and extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases and forming binding complexes, and uses for conducting nucleic acid sequencing reactions.