发明授权
US4220722A Method for conjugating to polyamino compounds employing haloacyl groups
and compositions prepared thereby
失效
使用卤代酰基与多氨基化合物共轭的方法和由此制备的组合物
- 专利标题: Method for conjugating to polyamino compounds employing haloacyl groups and compositions prepared thereby
- 专利标题(中): 使用卤代酰基与多氨基化合物共轭的方法和由此制备的组合物
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申请号: US876772申请日: 1978-02-10
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公开(公告)号: US4220722A公开(公告)日: 1980-09-02
- 发明人: Gerald L. Rowley , Danton Leung , Prithiphal Singh
- 申请人: Gerald L. Rowley , Danton Leung , Prithiphal Singh
- 申请人地址: CA Palo Alto
- 专利权人: Syva Company
- 当前专利权人: Syva Company
- 当前专利权人地址: CA Palo Alto
- 主分类号: G01N33/535
- IPC分类号: G01N33/535 ; C12N9/96
摘要:
Methods and compositions are provided for conjugating a wide variety of compounds, particularly polyfunctional compounds, having a mercapto group, either naturally present or synthetically introduced, to a polyamino compound, particularly a polypeptide (including proteins). The method employs a haloalkylcarbonyl compound, which is conjugated to one or more of the amino groups under mild acylating conditions. This is followed by combining the acylated polyamino compound with a mercapto containing compound, whereby the halogen is displaced by the sulfur of the mercapto group to form a stable thioether linkage. The resulting conjugates, depending on the compounds involved, can find uses in immunoassays, as hapten-antigen conjugates for the production of antibodies, and as ligand analog enzyme conjugates for use as reagents in controlling the distribution of substitution of a mercapto compound to a polyamino compound.
公开/授权文献
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