公开(公告)号：US4220722A

公开(公告)日：1980-09-02

申请号：US876772

申请日：1978-02-10

申请人： Gerald L. Rowley ,  Danton Leung ,  Prithiphal Singh

发明人： Gerald L. Rowley ,  Danton Leung ,  Prithiphal Singh

IPC分类号： G01N33/535 ,  C12N9/96

CPC分类号： G01N33/535 ,  Y10S435/961 ,  Y10S435/964 ,  Y10S436/816 ,  Y10S436/823 ,  Y10S530/806

摘要： Methods and compositions are provided for conjugating a wide variety of compounds, particularly polyfunctional compounds, having a mercapto group, either naturally present or synthetically introduced, to a polyamino compound, particularly a polypeptide (including proteins). The method employs a haloalkylcarbonyl compound, which is conjugated to one or more of the amino groups under mild acylating conditions. This is followed by combining the acylated polyamino compound with a mercapto containing compound, whereby the halogen is displaced by the sulfur of the mercapto group to form a stable thioether linkage. The resulting conjugates, depending on the compounds involved, can find uses in immunoassays, as hapten-antigen conjugates for the production of antibodies, and as ligand analog enzyme conjugates for use as reagents in controlling the distribution of substitution of a mercapto compound to a polyamino compound.

摘要翻译： 提供了将多种具有巯基的化合物，特别是多官能化合物，天然存在或合成引入的多种化合物，特别是多肽（包括蛋白质）共轭的方法和组合物。 该方法采用卤代烷基羰基化合物，其在温和的酰化条件下与一个或多个氨基相连。 然后将酰化的聚氨基化合物与含巯基的化合物结合，由此卤素被巯基的硫取代，形成稳定的硫醚键。 取决于所涉及的化合物，得到的缀合物可用于免疫测定中，作为产生抗体的半抗原共轭体，以及用作控制巯基化合物取代聚氨基酸的配体的配体类似物酶偶联物 复合。

公开(公告)号：US4328311A

公开(公告)日：1982-05-04

申请号：US125713

申请日：1980-02-28

申请人： Gerald L. Rowley ,  Danton Leung ,  Prithipal Singh

发明人： Gerald L. Rowley ,  Danton Leung ,  Prithipal Singh

IPC分类号： C12N9/96 ,  G01N33/535

CPC分类号： C12N9/96 ,  G01N33/535 ,  Y10S530/806

摘要： Methods and compositions are provided for conjugating a wide variety of compounds, particularly polyfunctional compounds, having a mercapto group, either naturally present or synthetically introduced, to a polyamino compound, particularly a polypeptide (including proteins). The method employs a haloalkyl-carbonyl compound, which is conjugated to one or more of the amino groups under mild acylating conditions. This is followed by combining the acylated polyamino compound with a mercapto containing compound, whereby the halogen is displaced by the sulfur of the mercapto group to form a stable thioether linkage. The resulting conjugates, depending on the compounds involved, can find uses in immunoassays, as hapten-antigen conjugates for the production of antibodies, and as ligand analog enzyme conjugates for use as reagents in controlling the distribution of substitution of a mercapto compound to a polyamino compound.

摘要翻译： 提供了将多种具有巯基的化合物，特别是多官能化合物，天然存在或合成引入的多种化合物，特别是多肽（包括蛋白质）共轭的方法和组合物。 该方法采用卤代烷基 - 羰基化合物，其在轻度酰化条件下与一个或多个氨基结合。 然后将酰化的聚氨基化合物与含巯基的化合物结合，由此卤素被巯基的硫取代，形成稳定的硫醚键。 取决于所涉及的化合物，得到的缀合物可用于免疫测定中，作为用于产生抗体的半抗原共轭体，以及用作控制巯基化合物取代聚氨基酸的配体的配体类似物酶缀合物 复合。