公开(公告)号：US20250020643A1

公开(公告)日：2025-01-16

申请号：US18890310

申请日：2024-09-19

Applicant: CELL IDX, INC. ,  THE UNVERSITY OF CHICAGO

Inventor： David A. SCHWARTZ ,  Stephen J. KRON

IPC: G01N33/547 ,  C12N9/04 ,  C12N9/08 ,  C12N9/16 ,  C12Q1/6804 ,  C12Q1/682 ,  G01N33/533 ,  G01N33/535 ,  G01N33/543

Abstract: The instant disclosure provides reagent compounds, and antibody and oligonucleotide reagents, for use in a variety of assays, including immunoassays and nucleic acid hybridizations. The reagent compounds comprise a bridging antigen or bridging oligonucleotide and a latent crosslinker moiety, such as a tyramide moiety. The bridging antigens are recognizable by the antibody of a corresponding antibody reagent with high affinity, and the bridging oligonucleotides are complementary to the oligonucleotide of a corresponding oligonucleotide reagent. The antibody reagents and oligonucleotide reagents also comprise a crosslinker activation agent, such as a peroxidase enzyme. Reaction of the reagent compounds with the crosslinker activation agent results in the amplification of signal in assays for target cellular markers, including cellular antigens and nucleic acids. Also provided are detectable antibodies specific for the bridging antigens, kits comprising the reagent compounds and antibody and oligonucleotide reagents, methods of signal amplification using the compounds and reagents of the disclosure, methods of preparation of the compounds and reagents, and compositions comprising the compounds and reagents.

公开(公告)号：US20240369552A1

公开(公告)日：2024-11-07

申请号：US18772362

申请日：2024-07-15

Applicant: MeMed Diagnostics Ltd.

Inventor： Eran EDEN ,  Kfir OVED ,  Assaf COHEN-DOTAN ,  Roy NAVON ,  Olga BOICO ,  Meital PAZ

IPC: G01N33/569 ,  A61P31/04 ,  A61P31/12 ,  G01N33/50 ,  G01N33/535 ,  G01N33/547 ,  G01N33/68

Abstract: Methods of determining infection type are disclosed. In one embodiment, the method comprises measuring the amount of TRAIL and/or IP10 no more than two days from symptom onset.

公开(公告)号：US20240361341A1

公开(公告)日：2024-10-31

申请号：US18627199

申请日：2024-04-04

Applicant: Jana Care, Inc.

Inventor： Daniel B. Lookadoo ,  Ashok A. Kumar ,  Jeremy E. Schonhorn ,  Christopher M. Uherek ,  Michal Depa

IPC: G01N33/70 ,  G01N33/535 ,  G01N33/68 ,  G01N35/00 ,  G16C20/10 ,  H04W84/18

CPC classification number: G01N33/70 ,  G01N33/535 ,  G01N33/6893 ,  G01N35/00069 ,  G16C20/10 ,  H04W84/18

Abstract: Platforms for enzymatic assays for biomarkers, including systems, methods, and measuring devices by which a biomarker, such as creatinine, is measured using a small amount of biological fluid, such as blood, plasma, or serum. The measuring device or biosensor can be a test strip including a layered active component assembly positioned between two outer layers which enables multi-step enzymatic reactions operating in kinetic and/or endpoint (in which the reaction is allowed to near completion), and generally includes multiple layers with primary enzyme(s), coupling enzyme(s), and reagents to produce an optical signal correlated to the concentration of a biomarker in the sample. The test strip can be read using a portable optical reader coupled to a smart phone or tablet.

公开(公告)号：US12031978B2

公开(公告)日：2024-07-09

申请号：US18043017

申请日：2021-08-24

Applicant: Aurangzeb Nafees Nagy

Inventor： Aurangzeb Nafees Nagy

IPC: G01N33/50 ,  G01N33/534 ,  G01N33/535 ,  G01N33/483

CPC classification number: G01N33/5091 ,  G01N33/5047 ,  G01N2333/165 ,  G01N2800/50

Abstract: A method for determining a susceptibility of a patient to infection by SARS-CoV-2 virus includes obtaining a tissue sample from the patient. The method also includes obtaining first cells from the tissue sample. The method further includes adding the first cells to second cells infected with the SARS-CoV-2 virus. Moreover, the method includes measuring a property of the second cells related to death of the second cells after adding the first cells to the second cells. In addition, the method includes calculating a susceptibility of the patient to infection by the SARS-CoV-2 virus using at least the property of the second cells.

公开(公告)号：US20240085424A1

公开(公告)日：2024-03-14

申请号：US18517143

申请日：2023-11-22

Applicant: CELL IDX, INC.

Inventor： David A. SCHWARTZ

IPC: G01N33/574 ,  C07K16/00 ,  C07K16/44 ,  G01N33/533 ,  G01N33/535 ,  G01N33/543

CPC classification number: G01N33/5743 ,  C07K16/00 ,  C07K16/44 ,  G01N33/533 ,  G01N33/535 ,  G01N33/54306 ,  G01N33/574 ,  G01N2474/20 ,  G01N2800/60

Abstract: The present disclosure provides high-performance immunoreagents for use in a variety of immunologic assays and other related techniques. The immunoreagents comprise a primary antibody and a bridging antigen, wherein the bridging antigen is recognized by a detectable secondary antibody with high affinity. Also provided are compositions comprising panels of immunoreagents specific for multiple different target antigens and compositions comprising pairs of primary immunoreagents and their complementary detectable secondary antibodies. The paired primary immunoreagents and secondary antibodies are useful in a variety of immunologic assays, particularly in highly multiplexed assays, where the structure of the bridging antigen is varied in tandem with variation in the detectable secondary antibody, such that a multiplicity of immunoreagents are provided that are capable of simultaneously detecting a multiplicity of target antigens in a single assay. Also provided are kits comprising the immunoreagents, methods of immunologic assay using the immunoreagents of the disclosure, and methods of preparation of the immunoreagents.

公开(公告)号：US20230384295A1

公开(公告)日：2023-11-30

申请号：US18234057

申请日：2023-08-15

Applicant: Ultivue, Inc.

Inventor： Mark Bobrow ,  Stephanie Rae Hennek ,  Mael Manesse

IPC: G01N33/535 ,  G01N33/542 ,  G01N33/543 ,  G01N33/58 ,  C12Q1/6804

CPC classification number: G01N33/535 ,  G01N33/542 ,  G01N33/54306 ,  C12Q2563/125 ,  C12Q1/6804 ,  G01N2333/908 ,  C12Q2537/143 ,  G01N33/581

Abstract: A method for testing a sample for the presence of one or more targets comprises multiplexed catalyzed reporter deposition (CARD) is provided.

公开(公告)号：US11761964B2

公开(公告)日：2023-09-19

申请号：US16749162

申请日：2020-01-22

Applicant: CYANAGEN S.r.l.

Inventor： Leopoldo Della Ciana ,  Lorenzo Biagini ,  Thomas Paul Jansen ,  Rossana Perciaccante ,  Manuela Vargiolu ,  Marina Eleonora Vettraino

IPC: G01N33/58 ,  C09K11/07 ,  G01N33/535

CPC classification number: G01N33/581 ,  C09K11/07 ,  G01N33/582 ,  C09K2211/1037 ,  G01N33/535

Abstract: A method for increasing the light emission produced by the chemiluminescent reaction of 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, a peroxidase enzyme or a conjugate thereof, an enhancer, a co-enhancer and a peroxide oxidizer, wherein the enhancer is an anionic N-alkylphenothiazine and the co-enhancer is selected from a 4-dialkylaminopyridine or an N-azole, and

wherein the method comprises the following steps:

i. realizing a chemiluminescent substrate by means of mixing together 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, the enhancer, the co-enhancer and the peroxide oxidizer, and
ii. adding the peroxidase enzyme or a conjugate thereof to the chemiluminescent substrate.

公开(公告)号：US20230194526A1

公开(公告)日：2023-06-22

申请号：US17860961

申请日：2022-07-08

Applicant: Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences

Inventor： QI WANG ,  LIYAN CAO ,  HAIXUE ZHENG ,  HONG TIAN ,  XIANGYU KONG ,  CONG YUAN ,  YUEYUE DUAN

IPC: G01N33/569 ,  C07K14/005 ,  C07K16/10 ,  C12N15/70 ,  G01N33/535 ,  G01N33/543 ,  G01N33/577

CPC classification number: G01N33/56983 ,  C07K14/005 ,  C07K16/10 ,  C12N15/70 ,  G01N33/535 ,  G01N33/54306 ,  G01N33/577 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/76 ,  C12N2800/101 ,  G01N2333/165 ,  G01N2469/20

Abstract: The disclosure belongs to the field of biotechnology, and in particular to a blocking ELISA kit for detecting an antibody to swine acute diarrhea syndrome coronavirus (SADS-CoV) N protein. The kit includes an enzyme plate coated with SADS-CoV N protein, an HRP-labeled mouse anti-SADS-CoV N protein monoclonal antibody (mAb), a positive serum control, and a negative serum control. The kit may detect positive sera diluted at 1:512, with no cross-reaction with positive sera against porcine epidemic diarrhea virus (PEDV), transmissible gasteroenteritis virus (TGEV) and porcine deltacoronavinis (PDCoV) etc., and the intrabatch and interbatch coefficient of variation is less than 10%. The comparison result with the indirect immunofluorescence test shows that the concordance rate of the blocking ELISA kit of the present disclosure is 99.6%, the Kappa value is 0.91, and the blocking ELISA method established by the present disclosure is highly consistent with IFA.

公开(公告)号：US11629183B2

公开(公告)日：2023-04-18

申请号：US16958823

申请日：2018-12-28

Applicant: University of Florida Research Foundation, Inc.

Inventor： Benoit Giasson ,  Todd Eliot Golde ,  Cara Louise Croft ,  Yona Levites ,  Brenda Dawn Moore

IPC: A61K39/395 ,  A61K39/00 ,  C07K16/18 ,  C07K16/00 ,  G01N33/53 ,  G01N33/68 ,  A61B5/00 ,  G01N33/533 ,  G01N33/535

Abstract: Disclosed herein are monoclonal antibodies targeting specific tau epitopes, particularly, microtubule binding domain region 2 or region 4. Also disclosed herein are antibodies or antigen binding fragments thereof that specifically recognize a tau epitope consisting of the amino acid sequence of SEQ ID NO: 9 or 11. Also disclosed herein are methods of detecting tau protein in a subject, comprising performing an assay using the antibodies or antigen binding fragments thereof on the subject or on a biological sample obtained from the subject. Assay kits containing the disclosed antibodies are also provided. Further, methods of treating or preventing a tauopathy in a subject by administering to the subject tau antibodies or antigen-binding fragments thereof are provided.

公开(公告)号：US11592422B2

公开(公告)日：2023-02-28

申请号：US17743951

申请日：2022-05-13

Applicant: EYE3CONCEPTS INC.

Inventor： Syed Rahin Ahmed ,  Greter Amelia Ortega Rodriguez ,  Satish Kumar Tuteja ,  Seshasai Srinivasan ,  Amin Reza Rajabzadeh

IPC: G01N27/48 ,  C12Q1/00 ,  C12Q1/28 ,  G01N27/327 ,  G01N33/535

Abstract: Nanozymes capped with a radical-scavenging capping agent are disclosed for use in biosensing assays with improved sensitivity. The radical-scavenging capping agent facilitates the capture and retention of one or more radicals for enhancing a catalytic reaction. In some example embodiments, the nanozyme capped by the radical-scavenging capping agent is capable of catalyzing the decomposition of hydrogen peroxide or molecular oxygen. The capped nanozymes may be incorporated with an electrode, such as the working electrode of an electrochemical sensor, for achieving enhanced catalytic activity and a lower limit of detection. In some example embodiments, the radical-scavenging capping agent is or includes thiocyanate. A rapid ethanol detection device and associated method are described in which the working electrode of an electrochemical sensor is modified by a peroxidase-mimetic nanozyme capped with a radical-scavenging capping agent for the enhanced generation of a reduction current associated with the decomposition of hydrogen peroxide.