发明授权
- 专利标题： Method for producing recombinant bacterial plasmids containing the coding sequences of higher organisms
- 专利标题（中）： 生产含有高等生物编码序列的重组细菌质粒的方法
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申请号： US301141申请日： 1981-09-11
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公开(公告)号： US4440859A公开(公告)日： 1984-04-03
- 发明人: William J. Rutter , Howard M. Goodman , Axel Ullrich , John Shine , John Chirgwin , Raymond Pictet
- 申请人： William J. Rutter , Howard M. Goodman , Axel Ullrich , John Shine , John Chirgwin , Raymond Pictet
- 申请人地址： CA Berkeley
- 专利权人： The Regents of the University of California
- 当前专利权人： The Regents of the University of California
- 当前专利权人地址： CA Berkeley
- 主分类号： C12N15/00
- IPC分类号： C12N15/00 ; C12N1/00 ; C12P19/34 ; C12P21/00 ; C12P21/04
摘要：
Microorganism having a gene derived from a higher organism is produced by isolating cells from a higher organism containing messenger RNA, extracting the messenger RNA, synthesizing a double stranded cDNA using the messenger RNA as a template, inserting the cDNA into a plasmid and transforming a microorganism with the resultant recombinant plasmid.
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