发明授权
- 专利标题: Biologically functional molecular chimeras
- 专利标题(中): 生物功能分子嵌合体
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申请号: US959288申请日: 1978-11-09
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公开(公告)号: US4468464A公开(公告)日: 1984-08-28
- 发明人: Stanley N. Cohen , Herbert W. Boyer
- 申请人: Stanley N. Cohen , Herbert W. Boyer
- 申请人地址: CA Stanford
- 专利权人: The Board of Trustees of The Leland Stanford Junior University
- 当前专利权人: The Board of Trustees of The Leland Stanford Junior University
- 当前专利权人地址: CA Stanford
- 主分类号: C12N15/00
- IPC分类号: C12N15/00 ; C12N1/00 ; C12N1/20 ; C12P21/00
摘要:
Method and compositions are provided for replication and expression of exogenous genes in microorganisms. Plasmids or virus DNA are cleaved to provide linear DNA having ligatable termini, which are bound to a gene having complementary termini, to provide a biologically functional replicon with a desired phenotypical property. The replicon is inserted into a microorganism cell by transformation. Isolation of the transformants provides cells for replication and expression of the DNA molecules present in the modified plasmid. The method provides a convenient and efficient way to introduce genetic capability into microorganisms for the production of nucleic acids are proteins, such as medically or commercially useful enzymes, which may have direct usefulness, or may find expression in the production of drugs, such as hormones, antibiotics, or the like, fixation of nitrogen, fermentation, utilization of specific feedstocks, or the like.The invention was supported by generous grants of NIH, NSF and the American Cancer Society.