发明授权
US4477571A Chimeric plasmids that replicate in bacteria and yeast and
microorganisms transformed therewith
失效
在细菌和酵母中复制的嵌合质粒和用其转化的微生物
- 专利标题: Chimeric plasmids that replicate in bacteria and yeast and microorganisms transformed therewith
- 专利标题(中): 在细菌和酵母中复制的嵌合质粒和用其转化的微生物
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申请号: US346258申请日: 1982-02-05
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公开(公告)号: US4477571A公开(公告)日: 1984-10-16
- 发明人: Shing Chang , James H. Meade
- 申请人: Shing Chang , James H. Meade
- 申请人地址: CA Emeryville
- 专利权人: Cetus Corporation
- 当前专利权人: Cetus Corporation
- 当前专利权人地址: CA Emeryville
- 主分类号: C12N1/18
- IPC分类号: C12N1/18 ; C12N1/20 ; C12N15/00 ; C12N15/68 ; C12N15/81 ; C12N1/00
摘要:
Chimeric plasmids capable of transforming bacteria and yeast are described. The plasmids carry the Cm (chloramphenicol resistance) gene and the Tc (tetracycline resistance) gene as selectable markers. The Cm gene allows the plasmids to be selected for in wild-type strains of the yeast Saccharomyces cerevisiae. The Tc gene allows heterologous genes cloned into the plasmids to be selected for in Escherichia coli bacteria.
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