发明授权
US4621048A Reagents containing an anti-ligand bound to an anti-enzyme and methods
for employing said reagents in an immunoassy
失效
含有与抗酶结合的抗配体的试剂和在免疫应用中使用所述试剂的方法
- 专利标题： Reagents containing an anti-ligand bound to an anti-enzyme and methods for employing said reagents in an immunoassy
- 专利标题（中）： 含有与抗酶结合的抗配体的试剂和在免疫应用中使用所述试剂的方法
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申请号： US588682申请日： 1984-03-12
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公开(公告)号： US4621048A公开(公告)日： 1986-11-04
- 发明人: Yoshihiro Ashihara , Hiromasa Suzuki , Yasushi Kasahara
- 申请人： Yoshihiro Ashihara , Hiromasa Suzuki , Yasushi Kasahara
- 申请人地址： JPX Tokyo
- 专利权人： Fujirebio Kabushiki Kaisha
- 当前专利权人： Fujirebio Kabushiki Kaisha
- 当前专利权人地址： JPX Tokyo
- 优先权： JPX58-38975 19830311; JPX58-51494 19830329; JPX58-51495 19830329
- 主分类号： G01N33/53
- IPC分类号： G01N33/53 ; G01N33/536 ; G01N33/542
摘要：
The invention relates to a method and novel reagents for detecting a ligand in a biological sample. In the method, the sample is combined with (i) an enzyme or an enzyme bound to a first macromolecular compound, (ii) substrate for the enzyme, and (iii) an antibody complex comprising anti-ligand bound to anti-enzyme and a second macromolecular compound bound to either one or both of the anti-ligand and anti-enzyme. The activity of the enzyme varies as a function of the unknown amount of ligand contained in the biological sample.
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