发明授权
- 专利标题: Method of performing assay for analyte in liquid medium
- 专利标题(中): 在液体介质中进行分析物测定的方法
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申请号: US692162申请日: 1985-01-17
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公开(公告)号: US4745077A公开(公告)日: 1988-05-17
- 发明人: John Holian , John C. Edwards , John K. Martin , Stephen A. Charles
- 申请人: John Holian , John C. Edwards , John K. Martin , Stephen A. Charles
- 申请人地址: GB2 Buckinghamshire
- 专利权人: Amersham International plc.
- 当前专利权人: Amersham International plc.
- 当前专利权人地址: GB2 Buckinghamshire
- 优先权: GBX8401368 19840119
- 主分类号: A61K39/00
- IPC分类号: A61K39/00 ; G01N33/543 ; G01N33/553 ; G01N33/533
摘要:
The invention concerns assays, such a immunoassays or two-site immunometric assays, performed using a labelled reagent and another reagent bound to magnetically attractable particles which are suspendable but insoluble in a liquid assay medium. After the labelled reagent has become partitioned between the liquid phase and the particles, in proportions which depend on the concentration of an analyte in a sample, the liquid phase is removed. Then the particles are re-suspended in another liquid medium, and the concentration of label observed. The method is particularly suitable for fluorescent and chemiluminescent' label systems, and can conveniently be performed in microtiter plates in which the wells are optically screened from one another, the observations being made from above or below the wells.