发明授权
US4975376A Class II restriction endonuclease KspI and a process for obtaining it
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II类限制性内切核酸酶KspI及其获得的过程
- 专利标题: Class II restriction endonuclease KspI and a process for obtaining it
- 专利标题(中): II类限制性内切核酸酶KspI及其获得的过程
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申请号: US331670申请日: 1989-03-30
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公开(公告)号: US4975376A公开(公告)日: 1990-12-04
- 发明人: Bryan J. Bolton , Michael Jarsch , Gudrun Schmitz , Christoph Kessler
- 申请人: Bryan J. Bolton , Michael Jarsch , Gudrun Schmitz , Christoph Kessler
- 申请人地址: DEX Mannheim
- 专利权人: Boehringer Mannheim GmbH
- 当前专利权人: Boehringer Mannheim GmbH
- 当前专利权人地址: DEX Mannheim
- 优先权: DEX3811278 19880402
- 主分类号: C12N9/16
- IPC分类号: C12N9/16 ; C12N9/22 ; C12N15/09 ; C12R1/01
摘要:
The present invention provides a restriction endonuclease which recognizes palindromic sequences ##STR1## where C* is methylated, and cleaves these sequences at the position indicated by the arrows. This endonuclease is preferably from a microorganism of the genus Kluyvera. The present invention also provides a process for obtaining this new restriction endonuclease and a method for using the endonuclease.
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