发明授权
- 专利标题: Human t-PA production using vectors coding DHFR protein
- 专利标题(中): 人类t-PA生产使用编码DHFR蛋白的载体
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申请号: US499209申请日: 1990-03-22
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公开(公告)号: US5010002A公开(公告)日: 1991-04-23
- 发明人: Arthur Levinson , Christian Simonsen , Elizabeth M. Yelverton
- 申请人: Arthur Levinson , Christian Simonsen , Elizabeth M. Yelverton
- 申请人地址: CA San Francisco
- 专利权人: Genentech, Inc.
- 当前专利权人: Genentech, Inc.
- 当前专利权人地址: CA San Francisco
- 主分类号: C12N9/06
- IPC分类号: C12N9/06 ; C12N9/72 ; C12N15/58 ; C12N15/85
摘要:
A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild type DHFR with methotrexate.
公开/授权文献
- US5818608A Quick response facsimile apparatus 公开/授权日:1998-10-06
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