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公开(公告)号:US5011795A
公开(公告)日:1991-04-30
申请号:US499201
申请日:1990-03-22
申请人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
发明人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
CPC分类号: C12N9/0028 , C12N15/85 , C12N9/6459 , C12Y304/21069 , C12N2800/108 , C12N2840/20
摘要: A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild type DHFR with methotrexate.
摘要翻译: 公开了一种在真核宿主细胞中产生组织纤溶酶原激活物(t-PA)的方法。 通过用甲氨蝶呤处理用突变体或野生型DHFR转化的培养物共同扩增t-PA基因,获得增强的t-PA产生水平。
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公开(公告)号:USH2181H1
公开(公告)日:2007-02-06
申请号:US07663244
申请日:1991-02-28
申请人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
发明人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
IPC分类号: C12N15/09
摘要: A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild-type DHFR with methotrexate.
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公开(公告)号:US5849574A
公开(公告)日:1998-12-15
申请号:US450874
申请日:1995-05-26
申请人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
发明人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
CPC分类号: C12N9/0028 , C12N15/85 , C12N9/6459 , C12Y304/21069 , C12N2800/108 , C12N2830/00 , C12N2830/55
摘要: A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild type DHFR with methotrexate.
摘要翻译: 公开了一种在真核宿主细胞中产生组织纤溶酶原激活物(t-PA)的方法。 通过用甲氨蝶呤处理用突变体或野生型DHFR转化的培养物共同扩增t-PA基因,获得增强的t-PA产生水平。
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公开(公告)号:US06261837B1
公开(公告)日:2001-07-17
申请号:US09105412
申请日:1998-06-26
申请人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
发明人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
IPC分类号: C12N516
CPC分类号: C12N9/0028 , C12N9/6459 , C12N15/85 , C12N2800/108 , C12N2830/00 , C12N2830/55 , C12Y304/21069
摘要: A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild type DHFR with methotrexate.
摘要翻译: 公开了一种在真核宿主细胞中产生组织纤溶酶原激活物(t-PA)的方法。 通过用甲氨蝶呤处理用突变体或野生型DHFR转化的培养物共同扩增t-PA基因,获得增强的t-PA产生水平。
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公开(公告)号:US5424198A
公开(公告)日:1995-06-13
申请号:US162354
申请日:1993-12-03
申请人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
发明人: Arthur D. Levinson , Diane Pennica , William J. Kohr , Gordon A. Vehar , David V. Goeddel , Elizabeth M. Yelverton , Christian C. Simonsen
CPC分类号: C12N9/0028 , C12N15/85 , C12N9/6459 , C12Y304/21069 , C12N2800/108 , C12N2830/00 , C12N2830/55
摘要: A method for producing tissue plasminogen activator (t-PA)in eukaroytic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild-type DHFR with methotrexate.
摘要翻译: 公开了一种用于在真核细胞宿主细胞中产生组织纤溶酶原激活物(t-PA)的方法。 通过用甲氨蝶呤处理用突变体或野生型DHFR转化的培养物共同扩增t-PA基因,获得增强的t-PA产生水平。
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公开(公告)号:US5010002A
公开(公告)日:1991-04-23
申请号:US499209
申请日:1990-03-22
CPC分类号: C12N9/0028 , C12N15/85 , C12N9/6459 , C12Y304/21069
摘要: A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild type DHFR with methotrexate.
摘要翻译: 公开了一种在真核宿主细胞中产生组织纤溶酶原激活物(t-PA)的方法。 通过用甲氨蝶呤处理用突变体或野生型DHFR转化的培养物共同扩增t-PA基因,获得增强的t-PA产生水平。
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