发明授权
- 专利标题： DNA sequences encoding yeast ubiquitin hydrolase
- 专利标题（中）： DNA序列编码YEAST UBIQUITIN HYDROLASE
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申请号： US284281申请日： 1988-12-14
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公开(公告)号： US5108919A公开(公告)日： 1992-04-28
- 发明人: Chung-Cheng Liu , Harvey I. Miller
- 申请人： Chung-Cheng Liu , Harvey I. Miller
- 申请人地址： CA South San Francisco
- 专利权人： Genentech, Inc.
- 当前专利权人： Genentech, Inc.
- 当前专利权人地址： CA South San Francisco
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C07K14/00 ; C07K14/64 ; C12N1/21 ; C12N9/60 ; C12N9/80 ; C12N15/55 ; C12N15/57 ; C12P21/00 ; C12R1/19
摘要：
A ubiquitin hydrolase is provided having a purity of at least 70% homogeneity based on the weight of the total protein in the composition. Also provided are DNA sequences encoding ubiquitin hydrolases, as well as expression systems for their recombinant production. Processes are provided for purification of a ubiquitin hydrolase from eukaryotes and for its use in recovering any desired polypeptide free from its fusion at its N-terminus with ubiquitin.
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