发明授权
US5234820A Exopeptidase catalyzed site-specific bonding of supports, labels and
bioactive agents to proteins
失效
外肽酶催化载体，标记和生物活性剂对蛋白质的位点特异性结合
- 专利标题： Exopeptidase catalyzed site-specific bonding of supports, labels and bioactive agents to proteins
- 专利标题（中）： 外肽酶催化载体，标记和生物活性剂对蛋白质的位点特异性结合
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申请号： US545915申请日： 1990-06-28
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公开(公告)号： US5234820A公开(公告)日： 1993-08-10
- 发明人: Fred W. Wagner , Thomas R. Coolidge , Sheldon M. Schuster , Jay Stout , Dwane E. Wylie , Klaus Breddam , William Lewis
- 申请人： Fred W. Wagner , Thomas R. Coolidge , Sheldon M. Schuster , Jay Stout , Dwane E. Wylie , Klaus Breddam , William Lewis
- 申请人地址： NE Lincoln NE Lincoln
- 专利权人： Board of Regents of the University of Nebraska,BioNebraska, Inc.
- 当前专利权人： Board of Regents of the University of Nebraska,BioNebraska, Inc.
- 当前专利权人地址： NE Lincoln NE Lincoln
- 主分类号： C07K1/107
- IPC分类号： C07K1/107 ; C07K16/40 ; C07K17/00 ; C12N11/00 ; G01N33/532 ; G01N33/543 ; G01N33/58
摘要：
An auxiliary substance such as a label, support, or bioactive agent is attached to a protein at a site that is remote from the active site of the protein by the use of exopeptidase and a nucleophile which is an amino acid, amino acid derivative, amine or alcohol. In one embodiment, the nucleophile is attached to the carboxy terminus of a protein by catalysis with exopeptidase to form an adduct and then the adduct or its combination with a linker arm is bound to the auxiliary substance. In another embodiment, the auxiliary substance or its combination with a linker arm is bound to the nucleophile to form an intermediate substance which is then coupled by catalysis with exopeptidase to the carboxy terminus of a protein.
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