-
1.发明授权Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment 失效使用碳酸酐酶作为亲和力结合片段纯化定向克隆肽
公开(公告)号：US5595887A
公开(公告)日：1997-01-21
申请号：US552810
申请日：1990-07-16
申请人： Thomas R. Coolidge , Fred Wagner , Gino van Heeke , Sheldon M. Schuster , Jay Stout , Dwane E. Wylie
发明人： Thomas R. Coolidge , Fred Wagner , Gino van Heeke , Sheldon M. Schuster , Jay Stout , Dwane E. Wylie
IPC分类号： C07K14/575 , C07K1/22 , C07K7/14 , C07K14/00 , C07K14/245 , C07K14/47 , C07K14/585 , C07K14/595 , C07K19/00 , C12N1/21 , C12N9/14 , C12N9/88 , C12N15/09 , C12N15/62 , C12P21/00 , C12R1/19 , C12P21/04 , C12P21/06
CPC分类号： C12N9/14 , C07K14/245 , C07K14/47 , C07K14/585 , C07K14/595 , C07K7/14 , C12N15/62 , C12N9/88 , C07K2319/00 , C07K2319/50 , C07K2319/75
摘要： Methods are presented for producing and purifying a variable fusion polypeptide which can be purified by affinity chromatography with the binding protein partner. The variable fusion polypeptide construct has tandem coupled segments containing one or more copies of a desired peptide linked to carbonic anhydrase as the purification binding protein. In the methods, the fusion protein is expressed in a recombinant host using a recombinant vector containing a gene encoding the fusion polypeptide. Then the expressed fusion polypeptide is purified by immobilized reversible inhibitor affinity chromatography. Finally, the purified fusion polypeptide is cleaved from the desired peptides by chemical or enzymatic means and the desired peptides purified with affinity chromatography.
摘要翻译： 提供了用于产生和纯化可通过用结合蛋白配偶体进行亲和层析纯化的可变融合多肽的方法。 可变融合多肽构建体具有串联耦合区段，其含有与碳酸酐酶连接的期望肽的一个或多个拷贝作为纯化结合蛋白。 在该方法中，使用含有编码融合多肽的基因的重组载体，在重组宿主中表达融合蛋白。 然后通过固定的可逆抑制剂亲和层析纯化表达的融合多肽。 最后，通过化学或酶法将纯化的融合多肽从期望的肽切割，并用亲和层析纯化所需的肽。
-
2.发明授权Sequential peptide and oligonucleotide syntheses using immunoaffinity techniques 失效使用免疫亲和技术的顺序肽和寡核苷酸合成
公开(公告)号：US5049656A
公开(公告)日：1991-09-17
申请号：US288009
申请日：1988-12-21
申请人： William Lewis , Jay Stout , Gino Van Heeke , Dwane E. Wylie , Sheldon M. Schuster , Fred W. Wagner
发明人： William Lewis , Jay Stout , Gino Van Heeke , Dwane E. Wylie , Sheldon M. Schuster , Fred W. Wagner
IPC分类号： A61K39/395 , A61K48/00 , C07H21/04 , C07K1/00 , C07K1/02 , C07K1/04 , C07K1/06 , C07K1/113 , C07K1/22 , C07K14/42 , C07K14/575 , C07K14/655 , C07K16/00 , C12N15/20
CPC分类号： C07K1/006 , C07K1/04 , C07K1/22 , Y10S435/803 , Y10S435/81 , Y10S436/824
摘要： The invention is directed to a method for purifying sequentially synthesized peptides and oligonucleotides by immunoaffinity techniques. Selected products are lapped with an antigenic capping agent and are conjugated with antibodies that are specific for the capping agent.
摘要翻译： 本发明涉及通过免疫亲和技术纯化顺序合成的肽和寡核苷酸的方法。 选择的产物用抗原封端剂研磨，并与针对封端剂特异的抗体缀合。
-
3.发明授权Exopeptidase catalyzed site-specific bonding of supports, labels and bioactive agents to proteins 失效外肽酶催化载体，标记和生物活性剂对蛋白质的位点特异性结合
公开(公告)号：US5741686A
公开(公告)日：1998-04-21
申请号：US316810
申请日：1994-10-03
申请人： Fred W. Wagner , Thomas R. Coolidge , Dwane E. Wylie , Sheldon M. Schuster , William Lewis , Jay Stout
发明人： Fred W. Wagner , Thomas R. Coolidge , Dwane E. Wylie , Sheldon M. Schuster , William Lewis , Jay Stout
IPC分类号： C07K17/06 , C07K1/107 , C07K14/575 , C07K16/00 , C07K16/40 , C07K17/00 , C07K19/00 , C12N11/00 , C12N11/02 , C12N11/06 , G01N33/532 , G01N33/543 , G01N33/58 , C12N9/96 , C07K1/13 , C08H1/00
CPC分类号： C07K1/1077 , C07K16/40 , C07K17/00 , C12N11/00 , G01N33/532 , G01N33/543 , G01N33/58 , Y10S530/811 , Y10S530/812 , Y10S530/815 , Y10S530/816
摘要： A method is provided for preparing a labeled protein, immobilized protein or protein-bioactive agent composition by attaching a label, support or bioactive agent to a protein by exopeptidase catalysis at a site that is remote from the active site of the protein. More specifically, an amine or alcohol group of an amino acid, amine or alcohol nucleophile is reacted by exopeptidase catalysis with a C-terminus carboxylic acid group of a protein such as an antibody, enzyme or hormone to couple the nucleophile to the protein to form an adduct, and the adduct is bound to an auxiliary substance such as a support, label or bioactive agent or its combination with a linker arm by reacting a reactive substituent of the nucleophile with a reactive group of the auxiliary substance. Alternatively, the nucleophile is bound to the auxiliary substance or its combination with a linker arm to form an intermediate, and the intermediate is coupled by exopeptidase catalysis to the protein. The exopeptidase may be a serine or cysteine exocarboxypeptidase.
摘要翻译： 提供了一种用于通过在远离蛋白质活性位点的位点处通过外肽酶催化将标记物，载体或生物活性剂附着到蛋白质来制备标记的蛋白质，固定的蛋白质或蛋白质 - 生物活性剂组合物的方法。 更具体地，氨基酸，胺或醇亲核试剂的胺或醇基团通过外肽酶催化与诸如抗体，酶或激素的蛋白质的C末端羧酸基团反应，以将亲核体偶联到蛋白质上形成 加合物，并且加合物通过将亲核试剂的反应性取代基与辅助物质的反应性基团反应而与辅助物质例如载体，标记或生物活性剂或其与连接臂的组合结合。 或者，亲核试剂与辅助物质或其与连接臂的组合结合以形成中间体，并且中间体通过外肽酶催化与蛋白质偶联。 外肽酶可以是丝氨酸或半胱氨酸外切羧肽酶。
-
4.发明授权Exopeptidase catalyzed site-specific bonding of supports, labels and bioactive agents to proteins 失效外肽酶催化载体，标记和生物活性剂对蛋白质的位点特异性结合
公开(公告)号：US5234820A
公开(公告)日：1993-08-10
申请号：US545915
申请日：1990-06-28
申请人： Fred W. Wagner , Thomas R. Coolidge , Sheldon M. Schuster , Jay Stout , Dwane E. Wylie , Klaus Breddam , William Lewis
发明人： Fred W. Wagner , Thomas R. Coolidge , Sheldon M. Schuster , Jay Stout , Dwane E. Wylie , Klaus Breddam , William Lewis
IPC分类号： C07K1/107 , C07K16/40 , C07K17/00 , C12N11/00 , G01N33/532 , G01N33/543 , G01N33/58
CPC分类号： C12N11/00 , C07K1/1077 , C07K16/40 , C07K17/00 , G01N33/532 , G01N33/543 , G01N33/58 , Y10S530/816
摘要： An auxiliary substance such as a label, support, or bioactive agent is attached to a protein at a site that is remote from the active site of the protein by the use of exopeptidase and a nucleophile which is an amino acid, amino acid derivative, amine or alcohol. In one embodiment, the nucleophile is attached to the carboxy terminus of a protein by catalysis with exopeptidase to form an adduct and then the adduct or its combination with a linker arm is bound to the auxiliary substance. In another embodiment, the auxiliary substance or its combination with a linker arm is bound to the nucleophile to form an intermediate substance which is then coupled by catalysis with exopeptidase to the carboxy terminus of a protein.
摘要翻译： 辅助物质如标记物，载体或生物活性剂通过使用外肽酶和亲核试剂（氨基酸，氨基酸衍生物，胺）与远离蛋白质活性位点的位点连接到蛋白质上 或酒精。 在一个实施方案中，亲核试剂通过使用外肽酶的催化与蛋白质的羧基末端连接以形成加合物，然后加合物或其与连接臂的组合与辅助物质结合。 在另一个实施方案中，辅助物质或其与连接臂的组合与亲核试剂结合以形成中间体物质，然后通过与外肽酶的催化偶联至蛋白质的羧基末端。
-
5.发明授权Sequential peptide and oligonucleotide syntheses using immunoaffinity techniques 失效使用免疫亲和技术的顺序肽和寡核苷酸合成
公开(公告)号：US5221736A
公开(公告)日：1993-06-22
申请号：US454372
申请日：1989-12-21
申请人： Thomas R. Coolidge , William Lewis , Sheldon M. Schuster , Dwane Wylie , Fred W. Wagner , Jay Stout , Gino van Heeke
发明人： Thomas R. Coolidge , William Lewis , Sheldon M. Schuster , Dwane Wylie , Fred W. Wagner , Jay Stout , Gino van Heeke
IPC分类号： A61K39/395 , A61K48/00 , C07H21/04 , C07K1/00 , C07K1/02 , C07K1/04 , C07K1/06 , C07K1/113 , C07K1/22 , C07K14/42 , C07K14/575 , C07K14/655 , C07K16/00 , C12N15/20
CPC分类号： C07K1/006 , C07K1/04 , C07K1/22 , Y10S435/803 , Y10S435/81 , Y10S436/824
摘要： The invention is directed to a method of purifying sequentially synthesized peptides and oligonucleotides by affinity techniques. Selected products are capped with and N-terminus capping agent for peptides or a 5'-terminus capping agents for oligonucleotides, and then bound with affinity agents that are selective for the corresponding capping agents.
摘要翻译： 本发明涉及通过亲和技术纯化顺序合成的肽和寡核苷酸的方法。 所选择的产物与肽封端的N-末端封端剂或寡核苷酸的5'-末端封端剂，然后与对相应的封端剂有选择性的亲和试剂结合。
-
公开(公告)号：US6127150A
公开(公告)日：2000-10-03
申请号：US927128
申请日：1997-09-05
申请人： Thomas R. Coolidge , Fred Wagner , Gino van Heeke , Sheldon M. Schuster , Jay Stout , Dwane E. Wylie
发明人： Thomas R. Coolidge , Fred Wagner , Gino van Heeke , Sheldon M. Schuster , Jay Stout , Dwane E. Wylie
IPC分类号： C07K14/575 , C07K1/22 , C07K7/14 , C07K14/00 , C07K14/245 , C07K14/47 , C07K14/585 , C07K14/595 , C07K19/00 , C12N1/21 , C12N9/14 , C12N9/88 , C12N15/09 , C12N15/62 , C12P21/00 , C12R1/19 , C12P21/06 , A61K38/24 , C07K1/00 , C12P21/04
CPC分类号： C12N9/14 , C07K14/245 , C07K14/47 , C07K14/585 , C07K14/595 , C07K7/14 , C12N15/62 , C12N9/88 , C07K2319/00 , C07K2319/50 , C07K2319/75
摘要： The present invention is directed to a protein purification construct having three tandem, coupled segments composed of a binding protein, an interconnecting linker and a variable fused polypeptide which incorporates the one or more copies of a product peptide. The binding protein is a mammalian or human carbonic anhydrase, or a modified version of the carbonic anhydrase. The protein purification construct may be employed in methods for expression of the product peptide in microbial and higher organism and for ligand immobilized affinity purification of the product peptide.
摘要翻译： 本发明涉及具有由结合蛋白，互连接头和可变融合多肽组成的三个串联耦合区段的蛋白质纯化构建体，其结合产物肽的一个或多个拷贝。 结合蛋白是哺乳动物或人类碳酸酐酶，或碳酸酐酶的修饰形式。 蛋白质纯化构建体可以用于在微生物和更高级生物体中表达产物肽的方法和用于产物肽的固定化亲和纯化的配体。
-
7.发明授权
公开(公告)号：US5639624A
公开(公告)日：1997-06-17
申请号：US266163
申请日：1994-06-27
CPC分类号： C07K16/44 , G01N33/84 , C07K2317/55
摘要： The invention is directed to monoclonal antibodies, their fragments, single chains and polypeptide mimics of their hypervariable regions which immunoreact with bare small moieties such as metallic cations the hybridomas for production of the monoclonal antibodies, immunogen compounds for developing the hybridomas, and methods for use of the monoclonal antibodies.
摘要翻译： 本发明涉及它们的高变区的单克隆抗体，其片段，单链和多肽模拟物，其与裸露的小部分例如金属阳离子免疫反应，用于产生单克隆抗体的杂交瘤，用于开发杂交瘤的免疫原化合物和使用方法 的单克隆抗体。
-
公开(公告)号：US5464759A
公开(公告)日：1995-11-07
申请号：US18100
申请日：1993-02-17
申请人： Thomas R. Coolidge , William Lewis , Sheldon M. Schuster , Jay Stout , Gino van Heeke , Dwane Wylie , Fred W. Wagner
发明人： Thomas R. Coolidge , William Lewis , Sheldon M. Schuster , Jay Stout , Gino van Heeke , Dwane Wylie , Fred W. Wagner
IPC分类号： A61K39/395 , A61K48/00 , C07H21/04 , C07K1/00 , C07K1/02 , C07K1/04 , C07K1/06 , C07K1/113 , C07K1/22 , C07K14/42 , C07K14/575 , C07K14/655 , C07K16/00 , C12N15/20 , C12P19/34 , C12Q1/68
CPC分类号： C07K1/006 , C07K1/04 , C07K1/22 , Y10S435/803 , Y10S435/81 , Y10S436/824
摘要： The invention is directed to a method of purifying sequentially synthesized peptides and oligonucleotides by affinity techniques. Selected products are capped with and N-terminus capping agent for peptides or a 5'-terminus capping agents for oligonucleotides, and then bound with affinity agents that are selective for the corresponding capping agents.
摘要翻译： 本发明涉及通过亲和技术纯化顺序合成的肽和寡核苷酸的方法。 所选择的产物与肽封端的N-末端封端剂或寡核苷酸的5'-末端封端剂，然后与对相应的封端剂有选择性的亲和试剂结合。
-
公开(公告)号：US5807695A
公开(公告)日：1998-09-15
申请号：US773688
申请日：1996-12-27
IPC分类号： C07K16/44 , G01N33/84 , G01N33/547 , G01N33/531
CPC分类号： C07K16/44 , G01N33/84 , C07K2317/55
摘要： The invention is directed to monoclonal antibodies, their fragments, single chains and polypeptide mimics of their hypervariable regions which immunoreact with bare small moieties such as metallic cations and small organic molecules, the hybridomas for production of the monoclonal antibodies, immunogen compounds for developing the hybridomas, and methods for use of the monoclonal antibodies.
摘要翻译： 本发明涉及与裸露小部分如金属阳离子和小有机分子免疫反应的其高变区的单克隆抗体，其片段，单链和多肽模拟物，用于产生单克隆抗体的杂交瘤，用于开发杂交瘤的免疫原化合物 以及使用单克隆抗体的方法。
-
10.发明授权
公开(公告)号：US5503987A
公开(公告)日：1996-04-02
申请号：US990543
申请日：1992-12-14
IPC分类号： C07K14/76 , C07K14/00 , C07K16/00 , C07K16/44 , C12N5/10 , C12N15/02 , C12P21/08 , C12R1/91 , G01N33/53 , G01N33/543 , G01N33/68 , G01N33/84 , G01N33/547 , G01N33/531
CPC分类号： C07K16/44 , G01N33/5308 , G01N33/54353 , G01N33/68 , G01N33/84 , C07K2317/55
摘要： The invention is directed to monoclonal antibodies which immunoreact with bare small moieties such as metallic cations and small organic molecules, the hybridomas for production of the monoclonal antibodies, immunogen compounds for developing the hybridomas, and methods for use of the monoclonal antibodies.
摘要翻译： 本发明涉及与裸露小部分例如金属阳离子和小有机分子免疫反应的单克隆抗体，用于产生单克隆抗体的杂交瘤，用于开发杂交瘤的免疫原化合物和用于单克隆抗体的方法。
-
-
-
-
-
-
-
-
-
搜索结果
11 条记录 (耗时 0.023 秒)
