发明授权
US5260197A L-fucose dehydrogenase, a process for production thereof, quantitative
assay for L-fucose using said enzyme and a kit for quantitative assay
失效
L-岩藻糖脱氢酶，其生产方法，使用所述酶定量测定L-岩藻糖和用于定量测定的试剂盒
- 专利标题： L-fucose dehydrogenase, a process for production thereof, quantitative assay for L-fucose using said enzyme and a kit for quantitative assay
- 专利标题（中）： L-岩藻糖脱氢酶，其生产方法，使用所述酶定量测定L-岩藻糖和用于定量测定的试剂盒
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申请号： US407148申请日： 1989-09-14
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公开(公告)号： US5260197A公开(公告)日： 1993-11-09
- 发明人: Tatsuo Horiuchi , Minoru Hiruma , Toshiyuki Suzuki
- 申请人： Tatsuo Horiuchi , Minoru Hiruma , Toshiyuki Suzuki
- 申请人地址： JPX Chiba
- 专利权人： Noda Institute for Scientific Research
- 当前专利权人： Noda Institute for Scientific Research
- 当前专利权人地址： JPX Chiba
- 优先权： JPX63-234745 19880921
- 主分类号： C12N9/04
- IPC分类号： C12N9/04 ; C12Q1/26 ; C12Q1/32 ; C12R1/38
摘要：
L-Fucose dehydrogenase having the following Physicochemical properties (1) and (2):(1) Action and substrate specificityIt withdraws hydrogen from L-fucose and converts into L-fuconolactone and at the same time, reduces coenzyme NADP.sup.+ to NADPH.(2) pH and stable pH rangeWhen Tris-imidazole-sodium acetate buffer solution is used, the optimum pH is in a range of 9.0 to 10.0 and the stable pH range is between 8.0 and 10.5.The L-fucose dehydrogenase utilizing NADP.sup.+ as coenzyme can be obtained by culturing L-fucose dehydrogenase-producing strain belonging to the genus Pseudomonas in a medium and collecting formed L-fucose dehydrogenase from the culture.L-Fucose can be quantitatively determined by reacting a sample containing L-fucose with L-fucose dehydrogenase requiring NADP.sup.+ as coenzyme and assaying NADPH produced.
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