发明授权
- 专利标题: Agents for the detection of substrates with hydrolase activity
- 专利标题(中): 用于检测具有水解酶活性的底物的试剂
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申请号: US927870申请日: 1992-08-10
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公开(公告)号: US5292669A公开(公告)日: 1994-03-08
- 发明人: Hans-Joachim Guder , Werner Guthlein , Wolfgang Weckerle , Johann Berger , Harvey Buck , Rupert Herrmann
- 申请人: Hans-Joachim Guder , Werner Guthlein , Wolfgang Weckerle , Johann Berger , Harvey Buck , Rupert Herrmann
- 申请人地址: DEX Mannheim
- 专利权人: Boehringer Mannheim GmbH
- 当前专利权人: Boehringer Mannheim GmbH
- 当前专利权人地址: DEX Mannheim
- 主分类号: C12Q1/00
- IPC分类号: C12Q1/00 ; C07D233/54 ; C07D233/64 ; C07D263/32 ; C07D277/20 ; C07D277/24 ; C07D277/28 ; C07D277/34 ; C07F9/6506 ; C07F9/653 ; C07F9/6539 ; C07H15/26 ; C07H17/00 ; C12Q1/34 ; C12Q1/42 ; C12Q1/44 ; C07D277/82 ; C07D233/56
摘要:
A method of detecting hydrolase activity using as substrates the compounds of the formula ##STR1## wherein each of R.sup.1, R.sup.2 and R.sup.3 is C.sub.1 -C.sub.4 alkyl, or phenyl optionally substituted in the meta- or para- position by C.sub.1 -C.sub.6 alkyl, C.sub.1 -C.sub.6 alkoxy or mono- or di-(C.sub.1 -C.sub.6)-alkylamino, or optionally substituted by an O-X group in which X is a glycosyl, phosphate or acyl moiety of a natural substrate of the corresponding glycosidase, phosphatase or esterase.