发明授权
- 专利标题： Media for normal human muscle satellite cells
- 专利标题（中）： 正常人类肌肉卫星细胞的培养基
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申请号： US928958申请日： 1992-08-12
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公开(公告)号： US5324656A公开(公告)日： 1994-06-28
- 发明人: Richard G. Ham , Judith A. St. Clair , Zetan Nie
- 申请人： Richard G. Ham , Judith A. St. Clair , Zetan Nie
- 申请人地址： CO Boulder
- 专利权人： University of Colorado Foundation, Inc.
- 当前专利权人： University of Colorado Foundation, Inc.
- 当前专利权人地址： CO Boulder
- 主分类号： C12N5/077
- IPC分类号： C12N5/077 ; C12N5/00 ; C12N5/06 ; C12N5/08
摘要：
An improved basal nutrient medium MCDB 120 for the clonal growth of human muscle satellite cells (HMSC) is described. In addition, serum-free supplements for the clonal growth of HMSC containing serum albumin, dexamethasone, fetuin fraction mitogen, epidermal growth factor and insulin are described. Combination of MCDB 120 and the serum-free supplement results in a serum-free medium for growth of HMSC, which can be transplanted to muscles of patients afflicted with muscle degenerative diseases such as muscular dystrophy. The serum-free supplement can also be used with other basal nutrient media such as MCDB 131M for growth of HMSC. Addition of 5% dialyzed fetal bovine serum to the serum-free media described results in semi-defined media which allows significantly improved growth of HMSC.