公开(公告)号：US5324656A

公开(公告)日：1994-06-28

申请号：US928958

申请日：1992-08-12

申请人： Richard G. Ham ,  Judith A. St. Clair ,  Zetan Nie

发明人： Richard G. Ham ,  Judith A. St. Clair ,  Zetan Nie

IPC分类号： C12N5/077 ,  C12N5/00 ,  C12N5/06 ,  C12N5/08

CPC分类号： C12N5/0659 ,  C12N2500/20 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/39

摘要： An improved basal nutrient medium MCDB 120 for the clonal growth of human muscle satellite cells (HMSC) is described. In addition, serum-free supplements for the clonal growth of HMSC containing serum albumin, dexamethasone, fetuin fraction mitogen, epidermal growth factor and insulin are described. Combination of MCDB 120 and the serum-free supplement results in a serum-free medium for growth of HMSC, which can be transplanted to muscles of patients afflicted with muscle degenerative diseases such as muscular dystrophy. The serum-free supplement can also be used with other basal nutrient media such as MCDB 131M for growth of HMSC. Addition of 5% dialyzed fetal bovine serum to the serum-free media described results in semi-defined media which allows significantly improved growth of HMSC.

摘要翻译： 描述了用于人类肌肉卫星细胞（HMSC）的克隆生长的改进的基础营养培养基MCDB 120。 此外，还描述了含有血清白蛋白，地塞米松，胎球蛋白级分裂原，表皮生长因子和胰岛素的HMSC的克隆生长的无血清补体。 MCDB 120和无血清补充物的组合导致HMSC生长的无血清培养基，其可以移植到患有肌肉退行性疾病如肌营养不良的患者的肌肉中。 无血清补充剂也可用于其他基础营养培养基如MCDB 131M用于HMSC的生长。 将5％透析的胎牛血清加入到所述无血清培养基中，得到半定义培养基，其允许显着改善HMSC的生长。