发明授权
US5364782A Mutant microbial .alpha.-amylases with increased thermal, acid and/or
alkaline stability
失效
具有增加的热，酸和/或碱稳定性的突变型微生物α-淀粉酶
- 专利标题： Mutant microbial .alpha.-amylases with increased thermal, acid and/or alkaline stability
- 专利标题（中）： 具有增加的热，酸和/或碱稳定性的突变型微生物α-淀粉酶
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申请号： US623953申请日： 1990-11-29
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公开(公告)号： US5364782A公开(公告)日： 1994-11-15
- 发明人: Wilhelmus J. Quax , Yves Laroche , Adrianus W. H. Vollebregt , Patrick Stanssens , Marc Lauwereys
- 申请人： Wilhelmus J. Quax , Yves Laroche , Adrianus W. H. Vollebregt , Patrick Stanssens , Marc Lauwereys
- 申请人地址： NLX Delft BEX Brussels
- 专利权人： Gist-Brocades N.V.,Plant Genetic Systems N.V.
- 当前专利权人： Gist-Brocades N.V.,Plant Genetic Systems N.V.
- 当前专利权人地址： NLX Delft BEX Brussels
- 优先权： EPX89201735 19890629
- 主分类号： C12P19/14
- IPC分类号： C12P19/14 ; C12N20060101 ; C12N1/21 ; C12N9/26 ; C12N9/28 ; C12N15/09 ; C12N15/56 ; C12N15/70 ; C12N15/75 ; C12R1/07 ; C12R1/10 ; C12R1/125 ; C12R1/19 ; C12S3/12 ; D06L1/14 ; D06M16/00
摘要：
Thermostable and acid stable .alpha.-amylases are provided as expression products of genetically engineered .alpha.-amylase genes isolated from microorganisms, preferably belonging to the class of Bacilli. Both chemical and enzymatic mutagenesis methods are e.g. the bisulphite method and enzymatic misincorporation on gapped heteroduplex DNA. The mutant .alpha.-amylases have superior properties, e.g. improved thermostability over a broad pH range, for industrial application in starch processing and textile desizing.
公开/授权文献
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