发明授权
- 专利标题： Detection of residual host cell DNA by PCR
- 专利标题（中）： 通过PCR检测残留宿主细胞DNA
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申请号： US990300申请日： 1992-11-30
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公开(公告)号： US5393657A公开(公告)日： 1995-02-28
- 发明人: Bruce W. Letwin , Melissa A. Jezuit
- 申请人： Bruce W. Letwin , Melissa A. Jezuit
- 申请人地址： MA Cambridge
- 专利权人： Genetics Institute, Inc.
- 当前专利权人： Genetics Institute, Inc.
- 当前专利权人地址： MA Cambridge
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
A method for detecting the presence of small mounts of contaminant DNA, present in an amount of at least about 0.1 picogram of DNA, in a protein product produced by recombinant DNA technology. The method comprises using oligonucleotides with sequences directed to those of repetitive host cell line DNA sequences, as primers in a PCR reaction. Also disclosed are kits for the detection of small amounts of contaminant DNA in a protein product.
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