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    BMP-11 compositions
    3.
    发明申请
    BMP-11 compositions 失效
    BMP-11组合物

    公开(公告)号：US20030166910A1

    公开(公告)日：2003-09-04

    申请号：US10188886

    申请日：2002-07-05

    申请人： Genetics Institute, Inc.

    发明人： John M. Wozney ,  Anthony J. Celeste

    IPC分类号： C07K014/51 ,  C07H021/04 ,  C12P021/02 ,  C12N005/06

    CPC分类号： A61K38/1875 ,  C07K14/51 ,  C07K14/575 ,  C07K2319/00 ,  C12N5/0619 ,  C12N2501/155 ,  Y10S930/12

    摘要： Purified BMP-11 proteins and processes for producing them are disclosed. Recombinant DNA molecules encoding the BMP-11 proteins are also disclosed. The proteins may be useful in regulating follicle stimulating hormone, such as for contraception. In addition, the proteins may be useful for the induction of bone, cartilage and/or other connective tissue.

    摘要翻译： 公开了纯化的BMP-11蛋白质及其生产方法。 还公开了编码BMP-11蛋白的重组DNA分子。 蛋白质可用于调节促卵泡激素，例如用于避孕。 此外，蛋白质可用于诱导骨，软骨和/或其它结缔组织。

    Bone morphogenetic protein-16 (BMP-16) compositions
    4.
    发明申请
    Bone morphogenetic protein-16 (BMP-16) compositions 失效
    骨形态发生蛋白-16（BMP-16）组合物

    公开(公告)号：US20030158379A1

    公开(公告)日：2003-08-21

    申请号：US09994177

    申请日：2001-11-26

    申请人： Genetics Institute, Inc.

    发明人： Anthony J. Celeste ,  Beth L. Murray

    IPC分类号： C07K014/51

    CPC分类号： C07K14/495 ,  A61K38/00 ,  C07K14/51 ,  C07K2319/00

    摘要： Purified BMP-16 proteins and processes for producing them are disclosed. DNA molecules encoding the BMP-16 proteins are also disclosed. The proteins may be used in the treatment of bone, cartilage, other connective tissue defects and disorders, including tendon, ligament and meniscus, in wound healing and related tissue repair, as well as for treatment of disorders and defects to tissues which include epidermis, nerve, muscle, including cardiac muscle, and other tissues and wounds, and organs such as liver, lung, cardiac, pancreas and kidney tissue. The proteins may also be useful for the induction of growth and/or differentiation of undifferentiated embryonic and stem cells.

    摘要翻译： 公开了纯化的BMP-16蛋白质及其生产方法。 还公开了编码BMP-16蛋白质的DNA分子。 蛋白质可以用于治疗伤口愈合和相关组织修复中的骨，软骨，其他结缔组织缺陷和障碍，包括腱，韧带和半月板，以及用于治疗包括表皮的组织的病症和缺陷， 神经，肌肉，包括心肌和其他组织和伤口，以及诸如肝，肺，心脏，胰腺和肾组织的器官。 蛋白质也可用于诱导未分化胚胎和干细胞的生长和/或分化。

    Isotope-coded ionization-enhancing reagents (ICIER) for high-throughput protein identification and quantitation using matrix-assisted laser desorption ionization mass spectrometry
    5.
    发明申请
    Isotope-coded ionization-enhancing reagents (ICIER) for high-throughput protein identification and quantitation using matrix-assisted laser desorption ionization mass spectrometry 失效
    使用基质辅助激光解吸电离质谱法进行高通量蛋白质鉴定和定量的同位素编码电离增强试剂（ICIER）

    公开(公告)号：US20030054570A1

    公开(公告)日：2003-03-20

    申请号：US10044708

    申请日：2001-10-22

    申请人： Genetics Institute, Inc.

    发明人： Yongchang Qiu ,  Jack H. Wang ,  Rodney M. Hewick

    IPC分类号： G01N033/543

    CPC分类号： G01N33/6848 ,  G01N33/6851 ,  G01N2458/15 ,  Y10T436/13 ,  Y10T436/24 ,  Y10T436/25125

    摘要： Arginine-containing cysteine-modifying compounds useful for MALDI-MS analysis of proteins are provided. These compounds termed isotope-coded ionization enhancement reagents (ICIER) can provide ionization enhancement in MALDI-MS, relative quantitation, and additional database searching constraints at the same time without any extra sample manipulation. More specifically, ICIER increase the ionization efficiency of cysteine-containing peptides by attachment of a guanidino functional group. ICIER also increase the overall hydrophilicity of these peptides due to the hydrophilic nature of ICIER and thus increase the percentage of recovery of these peptides during sample handling and processing such as in-gel digestion or liquid chromatography. Finally, a combination of both light and heavy ICIER provides an accurate way to obtain relative quantitation of proteins by MALDI-MS and additional database searching constraints (number of cysteine residues in every single peptide peak) to increase the confidence of protein identification by peptide mass mapping.

    摘要翻译： 提供了可用于蛋白质MALDI-MS分析的含精氨酸的半胱氨酸修饰化合物。 这些称为同位素编码电离增强试剂（ICIER）的化合物可以在MALDI-MS中提供电离增强，相对定量和额外的数据库搜索约束，同时没有任何额外的样品操作。 更具体地，ICIER通过连接胍基官能团来增加含半胱氨酸的肽的电离效率。 由于ICIER的亲水性，ICIER还增加了这些肽的整体亲水性，因此在样品处理和加工（如凝胶内消化或液相色谱）期间增加了这些肽的回收百分比。 最后，轻和重的ICIER的组合提供了通过MALDI-MS和附加的数据库搜索约束（每个肽峰中的半胱氨酸残基数）获得蛋白质的相对定量的精确方式，以增加肽质量的蛋白质鉴定的置信度 映射。

    Acid-labile isotope-coded extractant (ALICE) and its use in quantitative mass spectrometric analysis of protein mixtures
    8.
    发明申请
    Acid-labile isotope-coded extractant (ALICE) and its use in quantitative mass spectrometric analysis of protein mixtures 失效
    酸不稳定同位素编码提取物（ALICE）及其在蛋白质混合物的定量质谱分析中的应用

    公开(公告)号：US20020164809A1

    公开(公告)日：2002-11-07

    申请号：US10045170

    申请日：2001-10-22

    申请人： Genetics Institute, Inc.

    发明人： Yongchang Qiu ,  Jack H. Wang ,  Rodney M. Hewick

    IPC分类号： G01N033/00

    CPC分类号： G01N33/6848 ,  C07D405/12 ,  G01N30/461 ,  G01N30/463 ,  G01N30/468 ,  G01N30/7233 ,  G01N33/532 ,  G01N33/58 ,  G01N33/6803 ,  Y10T436/10 ,  Y10T436/105831 ,  Y10T436/13 ,  Y10T436/24 ,  Y10T436/25125 ,  B01D15/362 ,  B01D15/325

    摘要： The method of the invention provides novel compounds, termed acid-labile isotope-coded extractants (ALICE), for quantitative mass spectrometric analysis of protein mixtures. The compounds contain a thiol-reactive group that is used to capture cysteine-containing peptides from all peptide mixtures, an acid-labile linker, and a non-biological polymer. One of the two acid-labile linkers is isotopically labeled and therefore enables the direct quantitation of peptides/proteins through mass spectrometric analysis. Because no functional proteins are required to capture peptides, a higher percentage of organic solvent can be used to solubilize the peptides, particularly hydrophobic peptides, through the binding, washing and eluting steps, thus permitting much better recovery of peptides. Moreover, since the peptides are covalently linked to the non-biological polymer (ALICE), more stringent washing is allowed in order to completely remove non-specifically bound species. Finally, peptides captured by ALICE are readily eluted from the polymer support under mild acidic condition with high yield and permit the direct down stream mass spectrometric analysis without any further sample manipulation. In combination with our novel dual column two dimensional liquid chromatography-mass spectrometry (2D-LC-MS/MS) design, the ALICE procedure proves to a general approach for quantitative mass spectrometric analysis of protein mixtures with better dynamic range and sensitivity.

    摘要翻译： 本发明的方法提供了新的化合物，称为酸不稳定同位素编码的提取物（ALICE），用于蛋白质混合物的定量质谱分析。 该化合物含有硫醇反应性基团，其用于从所有肽混合物，酸不稳定接头和非生物聚合物捕获含半胱氨酸的肽。 两个酸不稳定接头之一是同位素标记的，因此能够通过质谱分析直接定量肽/蛋白质。 由于不需要功能性蛋白来捕获肽，所以可以使用较高百分比的有机溶剂来通过结合，洗涤和洗脱步骤来增溶肽，特别是疏水性肽，从而可以更好地回收肽。 此外，由于肽与非生物聚合物（ALICE）共价连接，所以允许更严格的洗涤以完全除去非特异性结合的物质。 最后，ALICE捕获的肽在温和的酸性条件下以高产率容易地从聚合物载体上洗脱，并允许直接的下游质谱分析，无需进一步的样品操作。 结合我们的双柱二维液相色谱 - 质谱（2D-LC-MS / MS）设计，ALICE方法证明了具有更好的动态范围和灵敏度的蛋白质混合物的定量质谱分析的一般方法。

    Receptor proteins
    9.
    发明申请
    Receptor proteins 失效
    受体蛋白

    公开(公告)号：US20020137133A1

    公开(公告)日：2002-09-26

    申请号：US09874628

    申请日：2001-06-05

    申请人： Genetics Institute, Inc.

    发明人： John M. Wozney ,  Anthony J. Celeste ,  R. Scott Thies ,  Noboru Yamaji

    IPC分类号： C07K014/705 ,  C07H021/04 ,  C12P021/02 ,  C12N005/06

    CPC分类号： C07K14/71 ,  A61K38/00 ,  C07K14/51

    摘要： Novel serine/threonine receptor proteins and BMP receptor proteins are disclosed, as well as DNA molecules encoding said proteins and methods of using the receptor proteins. Further disclosed are truncated BMP receptor proteins and molecules which act as ligands to said BMP receptor proteins.

    摘要翻译： 公开了新的丝氨酸/苏氨酸受体蛋白质和BMP受体蛋白质，以及编码所述蛋白质的DNA分子和使用受体蛋白质的方法。 还公开了截短的BMP受体蛋白质和作为所述BMP受体蛋白质的配体的分子。