A catheter check valve assembly has a body member with a wall defining a generally cylindrical chamber and a transverse wall having an aperture lying on the axis of such chamber. A duckbill valve and an end cap supporting a catheter are positioned on one side of the transverse wall. A separator has a major portion positioned in said cylindrical chamber and an elongated cylindrical probe extending from said major portion. The separator is axially moveable from a retracted position where the probe is out of contact with the duckbill valve to a forward position extending through and opening the duckbill valve. A trocar may be extended through the catheter check valve assembly when the separator is in the forward position.