发明授权
US5514538A Bicarbonate assay and phosphoenolpyruvate carboxylase enzyme from
hyphomicrobium
失效
碳酸氢盐测定和磷酸烯醇丙酮酸羧化酶从hyphomicrobium
- 专利标题： Bicarbonate assay and phosphoenolpyruvate carboxylase enzyme from hyphomicrobium
- 专利标题（中）： 碳酸氢盐测定和磷酸烯醇丙酮酸羧化酶从hyphomicrobium
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申请号： US40273申请日： 1993-03-30
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公开(公告)号： US5514538A公开(公告)日： 1996-05-07
- 发明人: Christopher T. Evans , Richard A. Wisdom
- 申请人： Christopher T. Evans , Richard A. Wisdom
- 申请人地址： GB2 Suffolk
- 专利权人： Genzyme Limited
- 当前专利权人： Genzyme Limited
- 当前专利权人地址： GB2 Suffolk
- 优先权： GBX9010359 19900509
- 主分类号： C12N9/88
- IPC分类号： C12N9/88 ; C12Q1/32 ; C12Q1/527 ; C12R1/01 ; C12Q1/00 ; C12Q1/48
摘要：
Phosphoenolpyruvate carboxylase (PEPC) characterized by a specific activity of at least 40 units/mg; and/or by no more than 50% loss of activity after 60 days in 167 mM Tricine/HCl buffer, pH 8.0, and 15 mM MgCl.sub.2 ; and/or, at pH 6.5, an activity of at least 40% relative to the activity at optimum pH is disclosed. Such enzymatic activity can be obtained from serine pathway methylotrophs such as Hyphomicorbium, and is very suitable for use in measuring bicarbonate in biological samples.
公开/授权文献
- USD379911S Press pin remover 公开/授权日：1997-06-17
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