Disclosed is a novel purified propolis-extract which contains the effective components of propolis in a readily absorbable- and utilizable-form in vivo, as well as having an absorbance ratio (A.sub.310 /A.sub.660) of higher than 4,000, having a relatively-high quality and a satisfactory handleability, color, flavor, taste and antiseptic activity, and being substantially free of resins, waxes and dark colored impurities. The purified propolis-extract is prepared by a process comprising decreasing a readily water-soluble organic solvent in its aqueous high-concentration solution containing a crude propolis-extract to a prescribed concentration to form an upper-layer liquid containing the effective components of propolis and a lower-layer liquid containing a viscous sediment, and separating and recoverying the upper-layer liquid.