发明授权
- 专利标题： Methods for coupled high temperatures reverse transcription and polymerase chain reactions
- 专利标题（中）： 用于偶联高温逆转录和聚合酶链反应的方法
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申请号： US449050申请日： 1995-05-24
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公开(公告)号： US5561058A公开(公告)日： 1996-10-01
- 发明人: David H. Gelfand , Thomas W. Myers , Christopher L. Sigua
- 申请人： David H. Gelfand , Thomas W. Myers , Christopher L. Sigua
- 申请人地址： NJ Nutley
- 专利权人： Hoffmann-La Roche Inc.
- 当前专利权人： Hoffmann-La Roche Inc.
- 当前专利权人地址： NJ Nutley
- 主分类号： C12N9/12
- IPC分类号： C12N9/12 ; C12N15/70 ; C12P19/34 ; C12Q1/68 ; C12Q1/70
摘要：
Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided. Reagents particularly suited for the methods of the present invention are provided.
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