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    Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
    6.
    发明授权
    Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity 有权
    具有减弱的3'-5'核酸外切酶活性的热稳定或热活性DNA聚合酶分子

    公开(公告)号：US07148049B2

    公开(公告)日：2006-12-12

    申请号：US10401403

    申请日：2003-03-26

    申请人： Nancy J. Schoenbrunner ,  Thomas W. Myers ,  David H. Gelfand

    发明人： Nancy J. Schoenbrunner ,  Thomas W. Myers ,  David H. Gelfand

    IPC分类号： C12N9/12

    CPC分类号： C12N9/1252 ,  C07K2319/00

    摘要： The present invention provides thermostable or thermoactive DNA polymerases with attenuated 3′-5′ exonuclease activity, methods for their synthesis, methods for their use, kits comprising the polymerases, nucleic acids encoding the polymerases and cells comprising such a nucleic acid. The DNA polymerases of the invention are useful in many recombinant DNA techniques, such as nucleic acid amplification by the polymerase chain reaction. The DNA polymerases of the invention allow higher fidelity replication and amplification of a template DNA sequence, allow less degradation of primers and/or more efficient use of deoxynucleotide triphosphates and are in general more efficient and less costly to make and use.

    摘要翻译： 本发明提供具有减弱的3'-5'核酸外切酶活性的热稳定或热活性DNA聚合酶，其合成方法，其使用方法，包含聚合酶的试剂盒，编码聚合酶的核酸和包含这种核酸的细胞。 本发明的DNA聚合酶可用于许多重组DNA技术，例如通过聚合酶链反应的核酸扩增。 本发明的DNA聚合酶允许模板DNA序列的更高保真度复制和扩增，允许较少的引物降解和/或更有效地使用脱氧核苷酸三磷酸酯，并且通常更有效并且成本低廉。

    DNA polymerases with increased 3′-mismatch discrimination
    7.
    发明授权
    DNA polymerases with increased 3′-mismatch discrimination 有权
    具有增加的3'-不匹配鉴别的DNA聚合酶

    公开(公告)号：US08765436B2

    公开(公告)日：2014-07-01

    申请号：US13180225

    申请日：2011-07-11

    申请人： Keith Bauer ,  Fred Reichert ,  Thomas W. Myers

    发明人： Keith Bauer ,  Fred Reichert ,  Thomas W. Myers

    IPC分类号： C12N9/00 ,  C12N15/54

    CPC分类号： C12N9/1252 ,  C12Q1/6806 ,  C12Y207/07007

    摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

    摘要翻译： 公开了相对于相应的未修饰的聚合酶具有增加的3'-错配鉴别的突变型DNA聚合酶。 突变型聚合酶可用于各种公开的引物延伸方法。 还公开了相关组合物，包括重组核酸，载体和宿主细胞，其可用于例如用于产生突变型DNA聚合酶。

    DNA polymerases with increased 3'-mismatch discrimination
    8.
    发明授权
    DNA polymerases with increased 3'-mismatch discrimination 有权
    具有增加的3'-不匹配鉴别的DNA聚合酶

    公开(公告)号：US08715991B2

    公开(公告)日：2014-05-06

    申请号：US13162684

    申请日：2011-06-17

    申请人： Fred Reichert ,  Keith Bauer ,  Thomas W. Myers

    发明人： Fred Reichert ,  Keith Bauer ,  Thomas W. Myers

    IPC分类号： C12N9/12 ,  C12N15/54

    CPC分类号： C12N9/1252 ,  C12N9/1276 ,  C12P19/34 ,  C12Y207/07007

    摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

    摘要翻译： 公开了相对于相应的未修饰的聚合酶具有增加的3'-错配鉴别的突变型DNA聚合酶。 突变型聚合酶可用于各种公开的引物延伸方法。 还公开了相关组合物，包括重组核酸，载体和宿主细胞，其可用于例如用于产生突变型DNA聚合酶。