发明授权
US5563057A Method for refolding misfolded enzymes with detergent and cyclodextrin
失效
用洗涤剂和环糊精重折叠错误折叠的酶的方法
- 专利标题： Method for refolding misfolded enzymes with detergent and cyclodextrin
- 专利标题（中）： 用洗涤剂和环糊精重折叠错误折叠的酶的方法
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申请号： US332188申请日： 1994-10-31
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公开(公告)号： US5563057A公开(公告)日： 1996-10-08
- 发明人: Samuel H. Gellman , David B. Rozema
- 申请人： Samuel H. Gellman , David B. Rozema
- 申请人地址： WI Madison
- 专利权人： Wisconsin Alumni Research Foundation
- 当前专利权人： Wisconsin Alumni Research Foundation
- 当前专利权人地址： WI Madison
- 主分类号： C07K1/113
- IPC分类号： C07K1/113 ; C12N9/88 ; C12N9/96 ; C07K1/14
摘要：
A method for refolding an enzyme from a misfolded configuration to a second native and active configuration is presented. The method comprises adding a linear alkyl detergent to a misfolded enzyme to form an enzyme-detergent complex. Then the enzyme-detergent complex is contacted with a cyclodextrin to allow the enzyme to assume a second active configuration. The misfolded enzyme may have been previously denatured by heat or by chemical means other than a detergent.
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