Invention Grant
US5578466A Recombinant co-expression system of protein disulfide isomerase gene,
yeast receptor protein ERD2 gene and a foreign product polypeptide
gene, and a process for producing the foreign polypeptide using such
system
失效
蛋白二硫键异构酶基因重组共表达系统，酵母受体蛋白ERD2基因和外来产物多肽基因，以及使用该系统产生外源多肽的方法
- Patent Title: Recombinant co-expression system of protein disulfide isomerase gene, yeast receptor protein ERD2 gene and a foreign product polypeptide gene, and a process for producing the foreign polypeptide using such system
- Patent Title (中): 蛋白二硫键异构酶基因重组共表达系统，酵母受体蛋白ERD2基因和外来产物多肽基因，以及使用该系统产生外源多肽的方法
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Application No.: US872673Application Date: 1992-04-17
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Publication No.: US5578466APublication Date: 1996-11-26
- Inventor: Toshiya Hayano , Setsuko Katoh , Nobuhiro Takahashi , Masanori Suzuki , Keiichi Honma
- Applicant: Toshiya Hayano , Setsuko Katoh , Nobuhiro Takahashi , Masanori Suzuki , Keiichi Honma
- Applicant Address: JPX Tokyo
- Assignee: Tonen Corporation
- Current Assignee: Tonen Corporation
- Current Assignee Address: JPX Tokyo
- Priority: JPX3-114074 19910418; JPX3-311601 19911030
- Main IPC: C07K14/395
- IPC: C07K14/395 ; C07K14/765 ; C12N9/90 ; C12N15/14 ; C12N15/61 ; C12N15/81 ; C12N15/90 ; C12N1/19 ; C12N15/62
Abstract:
The present invention is directed to a transformant comprising the following expression units in a co-expressible state:an expression unit containing a gene coding for a receptor protein ERD2 from yeast or analog thereof which is capable of binding to a protein localizing in endoplasmic reticulum and having a signal for staying therein;an expression unit containing a gene coding for said protein localizing in endoplasmic reticulum; andan expression unit containing a foreign gene coding for a polypeptide which is a subject of function of said protein localizing in endoplasmic reticulum, andto a transformant comprising, in a co-expressible state, a fusion gene which is composed of a DNA fragment coding for a human serum albumin prepro-sequence and a foreign gene coding for a useful polypeptide. The present invention is also directed to a process for producing said polypeptide by co-expressing said genes in said transformant such that the polypeptide is predominantly secreted out of the transformant cell. Consequently, the invention has an advantage of improving the productivity of said polypeptide.
Public/Granted literature
- US5800284A Golf ball Public/Granted day:1998-09-01
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