利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

72 条记录 (耗时 0.04 秒)

    Methods for increasing the resistance of plants to fungi by silencing the fungal SMT1-gene
    2.
    发明授权
    Methods for increasing the resistance of plants to fungi by silencing the fungal SMT1-gene 有权
    通过沉默真菌SMT1基因来增加植物对真菌的抗性的方法

    公开(公告)号：US08927812B2

    公开(公告)日：2015-01-06

    申请号：US13514693

    申请日：2010-12-06

    申请人： Holger Schultheiβ

    发明人： Holger Schultheiβ

    IPC分类号： C12N15/82 ,  C12N15/00 ,  C12N15/14 ,  A01H5/00 ,  C12N9/10

    CPC分类号： C12N15/8218 ,  C12N9/1007 ,  C12N9/107 ,  C12N15/8282

    摘要： The present invention relates to methods for generating or increasing resistance to at least one fungi, in particular soy bean rust, in a plant or a part of a plant by the expression of RNA which is at least partial complementary and/or identical to the STM1-gene, wherein the RNA is capable to provide ds-RNA and/or siRNA and/or miRNA. Moreover, the invention relates to respective plants, parts thereof and vector constructs capable to provide such RNA and the use of such vector constructs to provide fungal resistant plants.

    摘要翻译： 本发明涉及通过表达与STM1至少部分互补和/或相同的RNA来产生或增加植物或植物部分中至少一种真菌，特别是大豆锈病的抗性的方法 基因，其中RNA能够提供ds-RNA和/或siRNA和/或miRNA。 此外，本发明涉及能够提供这种RNA的相应植物，其部分和载体构建体以及这种载体构建体用于提供真菌抗性植物的用途。

    Process of high purity albumin production
    4.
    发明授权
    Process of high purity albumin production 有权
    高纯白蛋白生产工艺

    公开(公告)号：US07223561B2

    公开(公告)日：2007-05-29

    申请号：US10873504

    申请日：2004-06-22

    申请人： Andrew Robert Goodey ,  Darell Sleep ,  Hendrik Van Urk ,  Stephen Berezenko ,  John Rodney Woodrow ,  Richard Alan Johnson ,  Patricia Carol Wood ,  Stephen James Burton ,  Alan Victor Quirk

    发明人： Andrew Robert Goodey ,  Darell Sleep ,  Hendrik Van Urk ,  Stephen Berezenko ,  John Rodney Woodrow ,  Richard Alan Johnson ,  Patricia Carol Wood ,  Stephen James Burton ,  Alan Victor Quirk

    IPC分类号： C12N15/14 ,  C07K14/00

    CPC分类号： C07K14/70571 ,  C07K14/765 ,  C07K2319/10

    摘要： A process is provided for the preparation of albumin which has extremely low levels of or is essentially free of colorants, metal ions, human proteins, host proteins, fragments of albumin, polymers or aggregates of albumin and viruses, and which is essentially non-glycated, relatively high in free thiol and with an intact C-terminus. The process comprises passing albumin (preferably expressed and secreted by transformed yeast) through positive mode cation exchange and then positive mode anion exchange chromatography. Other steps may also be employed, ultrafiltration, gel permeation chromatography, affinity chromatography binding the albumin by using blue dyes) and affinity chromatography binding contaminants by using an aminophenylboronic acid resin. Elution of albumin, with a compound having affinity for albumin, from a material having no specific affinity for albumin is also disclosed, as is removal of ammonium ions with a counter-ion.

    摘要翻译： 提供了一种制备白蛋白的方法，其具有极低水平或基本上不含着色剂，金属离子，人蛋白质，宿主蛋白质，白蛋白片段，白蛋白和病毒的聚合物或聚集体，并且基本上未被糖化 ，游离硫醇相对较高，具有完整的C末端。 该方法包括使白蛋白（优选由转化的酵母表达和分泌）通过阳离子阳离子交换，然后进行阳性阴离子交换层析。 还可以采用其它步骤，超滤，凝胶渗透色谱法，通过使用蓝色染料结合白蛋白的亲和层析）和通过使用氨基苯基硼酸树脂的亲和层析结合污染物。 还公开了从对白蛋白没有特异性亲和力的物质洗脱白蛋白和具有对白蛋白的亲和性的化合物，以及用抗衡离子除去铵离子。

    Compositions and methods for insecticidal control of stinkbugs
    10.
    发明授权
    Compositions and methods for insecticidal control of stinkbugs 有权
    臭椿害虫防治的组成和方法

    公开(公告)号：US08872001B2

    公开(公告)日：2014-10-28

    申请号：US13152795

    申请日：2011-06-03

    申请人： Karen E. Broglie ,  David C. Cerf ,  Rafael Herrmann ,  Albert L. Lu ,  Brian McGonigle ,  James K. Presnail

    发明人： Karen E. Broglie ,  David C. Cerf ,  Rafael Herrmann ,  Albert L. Lu ,  Brian McGonigle ,  James K. Presnail

    IPC分类号： C12N15/113 ,  C12N15/05 ,  C12N15/14 ,  A01H5/00 ,  A01N57/16 ,  A01N65/00 ,  C12N15/82

    CPC分类号： C12N15/8286 ,  A01N57/16 ,  A01N65/00 ,  A01N65/44 ,  C12N15/113 ,  C12N15/8218 ,  C12N2310/14 ,  C12N2310/531 ,  Y02A40/162

    摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Pentatomidae plant pest or a N. viridula, Acrosternum hilare, Piezodorus guildini, and/or Halymorpha halys plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-292 or 302-304 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Pentatomidae. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

    摘要翻译： 提供了使用沉积元件的方法和组合物，当被害虫摄入时，例如五子科植物害虫或N. viridula，Acrosternum hilare，Piezodorus guildini和/或Halymorpha halys植物害虫，降低靶的表达 序列在害虫中。 在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。 本发明提供了SEQ ID NO：1-292或302-304中任一项所述的各种靶多核苷酸或其活性变体和片段，其中目标害虫中一个或多个序列的表达降低控制害虫（ 即具有杀虫活性）。 进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。 在具体实施方案中，害虫是五角科。 还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。