发明授权
- 专利标题: Indicator cell line for detecting RNA viruses and method therefor
- 专利标题(中): 用于检测RNA病毒的指示细胞系及其方法
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申请号: US171214申请日: 1993-12-21
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公开(公告)号: US5591579A公开(公告)日: 1997-01-07
- 发明人: Paul D. Olivo , Sondra Schlesinger
- 申请人: Paul D. Olivo , Sondra Schlesinger
- 申请人地址: MO St. Louis
- 专利权人: Washington University
- 当前专利权人: Washington University
- 当前专利权人地址: MO St. Louis
- 主分类号: C07K14/08
- IPC分类号: C07K14/08 ; C12N5/10 ; C12N15/09 ; C12Q1/34 ; C12Q1/66 ; C12Q1/68 ; C12Q1/70 ; G01N33/569 ; C12N5/16 ; C12N7/00 ; C12N15/00
摘要:
Cell lines and methods are disclosed for detecting the presence of RNA viruses in a specimen. The cell lines are stably transformed with a DNA molecule that includes a promoter capable of being recognized by the DNA dependent RNA polymerase of the cell capable of directing the transcription of a cDNA of a structurally defective RNA virus genome operably coupled to the promoter. The cDNA contains a structural coding sequence encoding a selected reporter gene product. The RNA molecules transcribed by the DNA dependent RNA polymerase are not capable of causing the translation of the reporter gene in the cell except when an active related virus that provides the necessary trans-acting enzymes to cause the increased replication of the RNA containing the reporter gene which is then translated into the reporter gene product is provided. Methods utilizing the cell lines of this invention to detect RNA viruses in a specimen by incubating the specimen with the cell line and assaying for expression of the reporter gene and a kit containing a supply of the cells and a supply of the reagents necessary for the detection of the reporter gene product are also provided.
公开/授权文献
- US4863536A Method of encapsulating an electrical components 公开/授权日:1989-09-05
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