An apparatus for arranging cells in a culture medium includes a substrate having patterns on a surface thereof, the patterns each having different adhesive property to cells, a container containing therein the substrate, cells and liquid culture medium for incubating the cells, and a shaker for shaking, rotating or oscillating the container. The apparatus assures that cells stick only to a pattern on which a material to which cells are easy to stick is applied, and do not stick to a pattern on which the material is not applied.