发明授权
US5595889A Process for integration of a chosen gene on the chromosome of a
bacterium using Mu transposons
失效
使用Mu转座子将所选择的基因整合到细菌的染色体上的过程
- 专利标题： Process for integration of a chosen gene on the chromosome of a bacterium using Mu transposons
- 专利标题（中）： 使用Mu转座子将所选择的基因整合到细菌的染色体上的过程
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申请号： US341460申请日： 1994-11-17
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公开(公告)号： US5595889A公开(公告)日： 1997-01-21
- 发明人: Fran.cedilla.ois Richaud , Bruno Jarry , Koichi Takinami , Osamu Kurahashi , Anne Beyou
- 申请人： Fran.cedilla.ois Richaud , Bruno Jarry , Koichi Takinami , Osamu Kurahashi , Anne Beyou
- 申请人地址： FRX Paris
- 专利权人： Eurolysine
- 当前专利权人： Eurolysine
- 当前专利权人地址： FRX Paris
- 优先权： FRX8802078 19880222
- 主分类号： C12P13/08
- IPC分类号： C12P13/08 ; C12N15/09 ; C12N15/52 ; C12N15/67 ; C12N15/70 ; C12N15/74 ; C12N15/90 ; C12P21/00 ; C12R1/19 ; C12N1/00 ; C12N15/00 ; C12P1/00 ; C12P21/02
摘要：
The present invention relates to a process for integration of a chosen gene or of a specific DNA molecule into the chromosome or episome of a bacterium by cloning the gene or DNA molecule into a defective transposon which is then integrated into the chromosomal or episomal DNA of the bacteria. The defective transposon is incapable of transposition autonomously but can be induced to transpose when properly complemented. The complementation to induce transposition can be limited so that the defective transposon produces a specific number of copies and thereafter is stable. The number of copies of the defective transposon produced during the period of transposition can be estimated by the level of expression of a marker gene contained within the defective transposon.
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