发明授权
US5612210A Process for producing 7.beta.-(4-carboxybutanamido)-cephalosporanic acid
acylase ("GA") enzyme
失效
生产7β-（4-羧基丁酰氨基） - 头孢烷酸酰基转移酶（“GA”）酶的方法
- 专利标题： Process for producing 7.beta.-(4-carboxybutanamido)-cephalosporanic acid acylase ("GA") enzyme
- 专利标题（中）： 生产7β-（4-羧基丁酰氨基） - 头孢烷酸酰基转移酶（“GA”）酶的方法
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申请号： US366457申请日： 1994-12-30
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公开(公告)号： US5612210A公开(公告)日： 1997-03-18
- 发明人: Wilhelmus Van der Goes , Antonella Bernardi , Aldo Bosetti , Pietro Cesti , Giuliana Franzosi
- 申请人： Wilhelmus Van der Goes , Antonella Bernardi , Aldo Bosetti , Pietro Cesti , Giuliana Franzosi
- 申请人地址： ITX Rome
- 专利权人： Ministero Dell'Universita' e Della Ricerca Scientifica e Tecnologica
- 当前专利权人： Ministero Dell'Universita' e Della Ricerca Scientifica e Tecnologica
- 当前专利权人地址： ITX Rome
- 优先权： ITXMI94A0031 19940114
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C12N1/21 ; C12N9/80 ; C12N9/86 ; C12N15/00 ; C12N15/31 ; C12N15/55 ; C12N15/70 ; C12P35/02 ; C12R1/19 ; C12R1/38 ; C12N15/72
摘要：
The present invention relates to a process for producing large amounts of 7.beta.-(4-carboxybutanamido)-cephalosporanic acid acylase ("GA") enzyme, which process consists in growing, under suitable culture conditions, a recombinant Escherichia coli K12 strain and subsequently extracting the enzyme from the resulting culture.Enzyme production is controlled by a particular expression system which can be induced by corn-steep liquor, an extremely low cost component of the culture medium.The enzyme can be used in the enzymatic preparation of 7-amino-cephalosporanic acid by starting from 7.beta.-(4-carboxybutanamido)-cephalosporanic acid.
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