发明授权
- 专利标题： Transposon-containing DNA cloning vector and uses thereof
- 专利标题（中）： 含有转座子的DNA克隆载体及其用途
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申请号： US403582申请日： 1995-03-14
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公开(公告)号： US5645991A公开(公告)日： 1997-07-08
- 发明人: Claire M. Berg , Douglas E. Berg , Gan Wang
- 申请人： Claire M. Berg , Douglas E. Berg , Gan Wang
- 申请人地址： CT Storrs MO St. Louis
- 专利权人： Univ. of Connecticut,Washington University
- 当前专利权人： Univ. of Connecticut,Washington University
- 当前专利权人地址： CT Storrs MO St. Louis
- 主分类号： C12N15/10
- IPC分类号： C12N15/10 ; C12N15/85 ; C12N15/00
摘要：
The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed.
公开/授权文献
- US5001863A Hinge system 公开/授权日：1991-03-26
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