发明授权
US5650298A Tight control of gene expression in eucaryotic cells by
tetracycline-responsive promoters
失效
通过四环素响应启动子对真核细胞中基因表达的严格控制
- 专利标题: Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
- 专利标题(中): 通过四环素响应启动子对真核细胞中基因表达的严格控制
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申请号: US260452申请日: 1994-06-14
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公开(公告)号: US5650298A公开(公告)日: 1997-07-22
- 发明人: Hermann Bujard , Manfred Gossen , Jochen G. Salfeld , Jeffrey W. Voss
- 申请人: Hermann Bujard , Manfred Gossen , Jochen G. Salfeld , Jeffrey W. Voss
- 申请人地址: DEX Ludwigshafen DEX Ludwigshafen
- 专利权人: BASF Aktiengesellschaft,Knoll Aktiengesellschaft
- 当前专利权人: BASF Aktiengesellschaft,Knoll Aktiengesellschaft
- 当前专利权人地址: DEX Ludwigshafen DEX Ludwigshafen
- 主分类号: A01K67/027
- IPC分类号: A01K67/027 ; A61K48/00 ; C07K14/00 ; C12N5/08 ; C12N5/10 ; C12N15/00 ; C12N15/09 ; C12N15/31 ; C12N15/62 ; C12N15/63 ; C12N15/85 ; C12R1/91 ; C12P21/00 ; C07H21/04
摘要:
Transgenic animals carrying two transgenes, the first coding for a transactivator fusion protein comprising a tet repressor and a polypeptide which directly or indirectly activates in eucaryotic cells, and the second comprising a gene operably linked to a minimal promoter operably linked to at least one tet operator sequence, are disclosed. Isolated DNA molecules (e.g., targeting vectors) for integrating a polynucleotide sequence encoding a transactivator of the invention at a predetermined location within a second target DNA molecule by homologous recombination are also disclosed. Transgenic animals having the DNA molecules of the invention integrated at a predetermined location in a chromosome by homologous recombination are also encompassed by the invention. Methods to regulate the expression of a tet operator linked-gene of interest by administering tetracycline or a tetracycline analogue to an animal of the invention are also disclosed. The regulatory system of the invention allows for conditional inactivation or modulation of expression of a gene of interest in a host cell or animal.
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