发明授权
- 专利标题： Sexing method of bovine embryos
- 专利标题（中）： 牛胚胎的性别方法
-
申请号： US458393申请日： 1995-06-02
-
公开(公告)号： US5661011A公开(公告)日： 1997-08-26
- 发明人: Toshiyuki Kudo , Yoshiaki Itagaki , Seiji Sato , Shizuyo Sutou , Toyoo Nakamura
- 申请人： Toshiyuki Kudo , Yoshiaki Itagaki , Seiji Sato , Shizuyo Sutou , Toyoo Nakamura
- 申请人地址： JPX
- 专利权人： Itoham Foods, Inc.
- 当前专利权人： Itoham Foods, Inc.
- 当前专利权人地址： JPX
- 优先权： JPX3-352032 19911213
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C12Q1/68 ; C12P19/34
摘要：
The present invention provides PCR primers with which sexing of bovine embryos can be easily attained and provides a practical, rapid and reliable method for determining the sex of bovine embryos using these primers. The methods for determining the sex of the bovine embryos are characterized by discriminating PCR products which are obtained by amplifying specific DNA sequences by PCR with pairs of male-specific and gender-neutral primers. These primers are derived from DNAs which specifically hybridize to the bovine male genome and from DNAs which gender-neutrally hybridize to both bovine male and female genomes.
信息查询
IPC分类:
