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公开(公告)号：US5661011A
公开(公告)日：1997-08-26
申请号：US458393
申请日：1995-06-02
申请人： Toshiyuki Kudo , Yoshiaki Itagaki , Seiji Sato , Shizuyo Sutou , Toyoo Nakamura
发明人： Toshiyuki Kudo , Yoshiaki Itagaki , Seiji Sato , Shizuyo Sutou , Toyoo Nakamura
CPC分类号： C12Q1/6879
摘要： The present invention provides PCR primers with which sexing of bovine embryos can be easily attained and provides a practical, rapid and reliable method for determining the sex of bovine embryos using these primers. The methods for determining the sex of the bovine embryos are characterized by discriminating PCR products which are obtained by amplifying specific DNA sequences by PCR with pairs of male-specific and gender-neutral primers. These primers are derived from DNAs which specifically hybridize to the bovine male genome and from DNAs which gender-neutrally hybridize to both bovine male and female genomes.
摘要翻译： 本发明提供可以容易地获得牛胚胎性别的PCR引物，并提供使用这些引物确定牛胚胎性别的实用，快速和可靠的方法。 用于确定牛胚胎性别的方法的特征在于区分通过用雄性特异性和性别中性引物的PCR扩增特异性DNA序列获得的PCR产物。 这些引物衍生自与牛雄性基因组特异性杂交的DNA，以及与牛雄性和雌性基因组性别中性杂交的DNA。
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公开(公告)号：US5461145A
公开(公告)日：1995-10-24
申请号：US984044
申请日：1992-12-02
申请人： Toshiyuki Kudo , Yoshiaki Itagaki , Seiji Sato , Shizuyo Sutou , Toyoo Nakamura
发明人： Toshiyuki Kudo , Yoshiaki Itagaki , Seiji Sato , Shizuyo Sutou , Toyoo Nakamura
CPC分类号： C12Q1/6879
摘要： The present invention provides PCR primers with which sexing of bovine embryos can be easily attained and provides a practical, rapid and reliable method for determining the sex of bovine embryos using these primers. The methods for determining the sex of the bovine embryos are characterized by discriminating PCR products which are obtained by amplifying specific DNA sequences by PCR with pairs of male-specific and gender-neutral primers. These primers are derived from DNAs which specifically hybridize to the bovine male genome and from DNAs which gender-neutrally hybridize to both bovine male and female genomes.
摘要翻译： 本发明提供可以容易地获得牛胚胎性别的PCR引物，并提供使用这些引物确定牛胚胎性别的实用，快速和可靠的方法。 用于确定牛胚胎性别的方法的特征在于区分通过用雄性特异性和性别中性引物的PCR扩增特异性DNA序列获得的PCR产物。 这些引物衍生自与牛雄性基因组特异性杂交的DNA，以及与牛雄性和雌性基因组性别中性杂交的DNA。
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