发明授权
- 专利标题： Nucleic acid sequences and expression systems for heparinase II and heparinase III derived from Flavobacterium heparinum
- 专利标题（中）： 肝素肝素肝素II和肝素酶III的核酸序列和表达系统
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申请号： US258639申请日： 1994-06-10
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公开(公告)号： US5681733A公开(公告)日： 1997-10-28
- 发明人: Hongsheng Su , Francoise Blain , Clark Bennett , Kangfu Gu , Joseph Zimmermann , Roy Musil
- 申请人： Hongsheng Su , Francoise Blain , Clark Bennett , Kangfu Gu , Joseph Zimmermann , Roy Musil
- 申请人地址： CAX Montreal
- 专利权人： Ibex Technologies
- 当前专利权人： Ibex Technologies
- 当前专利权人地址： CAX Montreal
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C07K16/40 ; C12N1/21 ; C12N9/88 ; C12N15/60 ; C12N15/74 ; C12P21/08 ; C12R1/19
摘要：
The present invention describes the isolation and sequence of genes from Flavobacterium heparinum encoding heparin and heparan sulfate degrading enzymes, heparinase II and heparinase III (EC 4.2.2.8). It further describes a method of expressing and an expression for heparinases I, II and III using a modified ribosome binding region derived from a promoter from glycosaminoglycan lyase genes of F. heparinum. Also, a multi-step protein purification method incorporating cell disruption, cation exchange chromatography, affinity chromatography and hydroxylapatite chromatography is outlined. Antibodies against a post-translational modification moiety common to Flavobacterium heparinum proteins and a method to obtain antibodies specific to these moieties and to the amino acid sequences of heparinases I, II and III are described.
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